Protein kinase Cα-responsive polymeric carrier: Its application for gene delivery into human cancers

Tetsuro Tomiyama, Jeong Hun Kang, Riki Toita, Takuro Niidome, Yoshiki Katayama

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    For cancer-targeting gene delivery, we applied a protein kinase C (PKC)α-responsive polymeric carrier to human cancers (U-87 MG [human glioblastoma-astrocytoma, epithelial-like cell line]and A549 [human lung adenocarcinoma epithelial cell line]). Two polymers, one a PKCα-responsive polymer (PPC[S]) containing the phosphorylation site serine, and the other a negative control polymer (PPC[A]), in which the serine was substituted with alanine, were synthesized. No cytotoxicity of the polymer was identified. When the complexes were transfected into cancer cells or tissues in which PKCα was hyper-activated, the luciferase expression from the PPC(S)/plasmid (pDNA) complex was higher than that from the PPC(A)/pDNA complex. These results show that the phosphorylation of complex by PKCα in cancer cells leads to high gene expression and that our system can be used as a human cancer cell-targeting gene delivery system.

    Original languageEnglish
    Pages (from-to)1532-1536
    Number of pages5
    JournalCancer Science
    Volume100
    Issue number8
    DOIs
    Publication statusPublished - Jul 23 2009

    All Science Journal Classification (ASJC) codes

    • Oncology
    • Cancer Research

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