Protein kinase Cα-responsive polymeric carrier: Its application for gene delivery into human cancers

Tetsuro Tomiyama, Jeong Hun Kang, Riki Toita, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

For cancer-targeting gene delivery, we applied a protein kinase C (PKC)α-responsive polymeric carrier to human cancers (U-87 MG [human glioblastoma-astrocytoma, epithelial-like cell line]and A549 [human lung adenocarcinoma epithelial cell line]). Two polymers, one a PKCα-responsive polymer (PPC[S]) containing the phosphorylation site serine, and the other a negative control polymer (PPC[A]), in which the serine was substituted with alanine, were synthesized. No cytotoxicity of the polymer was identified. When the complexes were transfected into cancer cells or tissues in which PKCα was hyper-activated, the luciferase expression from the PPC(S)/plasmid (pDNA) complex was higher than that from the PPC(A)/pDNA complex. These results show that the phosphorylation of complex by PKCα in cancer cells leads to high gene expression and that our system can be used as a human cancer cell-targeting gene delivery system.

Original languageEnglish
Pages (from-to)1532-1536
Number of pages5
JournalCancer Science
Volume100
Issue number8
DOIs
Publication statusPublished - Jul 23 2009

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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