Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells

Yoshinori Katakura, Miyako Udono, Kazuyuki Katsuki, Hisaya Nishide, Yukiko Tabira, Takahiro Ikei, Makiko Yamashita, Tsukasa Fujiki, Sanetaka Shirahata

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In the present study, we clarified that transforming growth factor β (TGF-β) induces cellular senescence in human normal diploid cells, TIG-1, and identified protein kinase Cs (PKCs) as downstream mediators of TGF-β-induced cellular senescence. Among PKCs, we showed that PKC-δ induced cellular senescence in TIG-1 cells and was activated in replicatively and prematurely senescent TIG-1 cells. The causative role of PKC-δ in cellular senescence programs was demonstrated using a kinase negative PKC-δ and small interfering RNA against PKC-δ. Furthermore, PKC-δ was shown to function in human telomerase reverse transcriptase (hTERT) gene repression. These results indicate that PKC-δ plays a key role in cellular senescence programs, and suggest that the induction of senescence and hTERT repression are coordinately regulated by PKC-δ.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalJournal of biochemistry
Volume146
Issue number1
DOIs
Publication statusPublished - Jul 1 2009

Fingerprint

Cell Aging
Diploidy
Protein Kinases
Protein Kinase C
Transforming Growth Factors
Small Interfering RNA
Phosphotransferases
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells. / Katakura, Yoshinori; Udono, Miyako; Katsuki, Kazuyuki; Nishide, Hisaya; Tabira, Yukiko; Ikei, Takahiro; Yamashita, Makiko; Fujiki, Tsukasa; Shirahata, Sanetaka.

In: Journal of biochemistry, Vol. 146, No. 1, 01.07.2009, p. 87-93.

Research output: Contribution to journalArticle

Katakura, Y, Udono, M, Katsuki, K, Nishide, H, Tabira, Y, Ikei, T, Yamashita, M, Fujiki, T & Shirahata, S 2009, 'Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells' Journal of biochemistry, vol. 146, no. 1, pp. 87-93. https://doi.org/10.1093/jb/mvp046
Katakura, Yoshinori ; Udono, Miyako ; Katsuki, Kazuyuki ; Nishide, Hisaya ; Tabira, Yukiko ; Ikei, Takahiro ; Yamashita, Makiko ; Fujiki, Tsukasa ; Shirahata, Sanetaka. / Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells. In: Journal of biochemistry. 2009 ; Vol. 146, No. 1. pp. 87-93.
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