TY - JOUR
T1 - Protein-protein interactions in the subunits of ribonuclease P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3
AU - Kifusa, Mayumi
AU - Fukuhara, Hideo
AU - Hayashi, Takeshi
AU - Kimura, Makoto
N1 - Funding Information:
We thank Dr. Takeshi Yamagami of Kyushu University for helpful suggestions as to the yeast two-hybrid assay. This work was supported in part by a grant from the National Project on Protein Structural and Functional Analyses.
PY - 2005/6
Y1 - 2005/6
N2 - Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5′ leader sequence of precursor tRNA (pre-tRNA). RNase P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 consists of RNA and five protein subunits (Ph1481p, Ph1496p, Ph1601p, Ph1771p, and Ph1877p). In vivo interactions among five protein subunits of RNase P in P. horikoshii OT3 were examined using a yeast two-hybrid system. The analysis indicates that proteins Ph1481p and Ph1601p interact strongly with Ph1877p and Ph1771p respectively, whereas Ph1481p interacts moderately with Ph1601p. In contrast, no interaction was detected between Ph1496p and the other four proteins. Co-immunoprecipitation analysis confirmed the interactions obtained by yeast two-hybrid assay.
AB - Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5′ leader sequence of precursor tRNA (pre-tRNA). RNase P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 consists of RNA and five protein subunits (Ph1481p, Ph1496p, Ph1601p, Ph1771p, and Ph1877p). In vivo interactions among five protein subunits of RNase P in P. horikoshii OT3 were examined using a yeast two-hybrid system. The analysis indicates that proteins Ph1481p and Ph1601p interact strongly with Ph1877p and Ph1771p respectively, whereas Ph1481p interacts moderately with Ph1601p. In contrast, no interaction was detected between Ph1496p and the other four proteins. Co-immunoprecipitation analysis confirmed the interactions obtained by yeast two-hybrid assay.
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U2 - 10.1271/bbb.69.1209
DO - 10.1271/bbb.69.1209
M3 - Article
C2 - 15973057
AN - SCOPUS:22444431914
SN - 0916-8451
VL - 69
SP - 1209
EP - 1212
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 6
ER -