Protein-protein interactions in the subunits of ribonuclease P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3

Mayumi Kifusa, Hideo Fukuhara, Takeshi Hayashi, Makoto Kimura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5′ leader sequence of precursor tRNA (pre-tRNA). RNase P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 consists of RNA and five protein subunits (Ph1481p, Ph1496p, Ph1601p, Ph1771p, and Ph1877p). In vivo interactions among five protein subunits of RNase P in P. horikoshii OT3 were examined using a yeast two-hybrid system. The analysis indicates that proteins Ph1481p and Ph1601p interact strongly with Ph1877p and Ph1771p respectively, whereas Ph1481p interacts moderately with Ph1601p. In contrast, no interaction was detected between Ph1496p and the other four proteins. Co-immunoprecipitation analysis confirmed the interactions obtained by yeast two-hybrid assay.

Original languageEnglish
Pages (from-to)1209-1212
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number6
DOIs
Publication statusPublished - Jun 1 2005

Fingerprint

Pyrococcus horikoshii
Ribonuclease P
Archaea
Two-Hybrid System Techniques
Protein Subunits
Yeast
Proteins
Ribonucleoproteins
RNA Precursors
Hybrid systems
Immunoprecipitation
Assays
RNA
Processing

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Protein-protein interactions in the subunits of ribonuclease P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3. / Kifusa, Mayumi; Fukuhara, Hideo; Hayashi, Takeshi; Kimura, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 6, 01.06.2005, p. 1209-1212.

Research output: Contribution to journalArticle

Kifusa, Mayumi ; Fukuhara, Hideo ; Hayashi, Takeshi ; Kimura, Makoto. / Protein-protein interactions in the subunits of ribonuclease P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3. In: Bioscience, Biotechnology and Biochemistry. 2005 ; Vol. 69, No. 6. pp. 1209-1212.
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