Protein secondary structure imaging with ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy

Kotatsu Bito, Masanari Okuno, Hideaki Kano, Shihomi Tokuhara, Satoru Naito, Yoshinori Masukawa, Philippe Leproux, Vincent Couderc, Hiro O. Hamaguchi

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Protein secondary structures in human hair have been studied with ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The CARS peak-shift mapping method has been developed and applied to hair samples with and without treatments by chemical reduction and mechanical extension. It clearly visualizes the treatment induced changes in protein secondary structures and their spatial distributions. Using the new imaging technique, we found a multilayered structure in the human hair cortex.

Original languageEnglish
Pages (from-to)1452-1457
Number of pages6
JournalJournal of Physical Chemistry B
Volume116
Issue number4
DOIs
Publication statusPublished - Feb 2 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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