Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals

Hirofumi Kaneko, Junichi Kamiie, Hirotaka Kawakami, Takahisa Anada, Yoshitomo Honda, Naru Shiraishi, Shinji Kamakura, Tetsuya Terasaki, Hidetoshi Shimauchi, Osamu Suzuki

Research output: Contribution to journalArticle

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Abstract

The present study was designed to determine which proteins are selectively adsorbed onto two bone substitute materials, octacalcium phosphate (OCP) and hydroxyapatite (HA) crystals, from rat serum by proteome analysis. Ground crystals of synthetic OCP and commercially available sintered HA, with the same surface area, were incubated in rat serum proteins at 37 °C for 24 h. The proteins from the crystals extracted with guanidine-HCl-EDTA were listed on the basis of the results of liquid chromatography tandem mass spectrometry (LC/MS/MS). A total of 138 proteins were detected from OCP; 103 proteins were detected from HA. Forty-eight proteins were from both crystals. A quantitative analysis of the proteins detected was performed for the extracted two bone formation-related proteins apolipoprotein E (Apo E), a protein known to promote osteoblast differentiation, and complement 3 (C3). HA adsorbed C3 (3.98 ± 0.03 fmol/μg protein) more than OCP (1.81 ± 0.07 fmol/μg protein) did, while OCP adsorbed Apo E (2.42 ± 0.03 fmol/μg protein) more than HA (1.21 ± 0.01 fmol/μg protein) did even after deleting the high-abundance proteins, such as albumin. The results demonstrated that OCP exhibits a similar property but distinct capacity with HA in adsorbing bone formation-related proteins from the serum constituents.

Original languageEnglish
Pages (from-to)276-285
Number of pages10
JournalAnalytical Biochemistry
Volume418
Issue number2
DOIs
Publication statusPublished - Nov 15 2011

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Proteome
Rats
Blood Proteins
Crystals
Durapatite
Proteins
Complement C3
Apolipoproteins E
Osteogenesis
octacalcium phosphate
Bone
Bone Substitutes
Osteoblasts
Guanidine
Liquid chromatography
Tandem Mass Spectrometry
Edetic Acid
Liquid Chromatography
Mass spectrometry
Albumins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals. / Kaneko, Hirofumi; Kamiie, Junichi; Kawakami, Hirotaka; Anada, Takahisa; Honda, Yoshitomo; Shiraishi, Naru; Kamakura, Shinji; Terasaki, Tetsuya; Shimauchi, Hidetoshi; Suzuki, Osamu.

In: Analytical Biochemistry, Vol. 418, No. 2, 15.11.2011, p. 276-285.

Research output: Contribution to journalArticle

Kaneko, H, Kamiie, J, Kawakami, H, Anada, T, Honda, Y, Shiraishi, N, Kamakura, S, Terasaki, T, Shimauchi, H & Suzuki, O 2011, 'Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals', Analytical Biochemistry, vol. 418, no. 2, pp. 276-285. https://doi.org/10.1016/j.ab.2011.07.022
Kaneko, Hirofumi ; Kamiie, Junichi ; Kawakami, Hirotaka ; Anada, Takahisa ; Honda, Yoshitomo ; Shiraishi, Naru ; Kamakura, Shinji ; Terasaki, Tetsuya ; Shimauchi, Hidetoshi ; Suzuki, Osamu. / Proteome analysis of rat serum proteins adsorbed onto synthetic octacalcium phosphate crystals. In: Analytical Biochemistry. 2011 ; Vol. 418, No. 2. pp. 276-285.
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