Proteome analysis of silk gland proteins from the silkworm, Bombyx mori

Pingbo Zhang, Yoichi Aso, Kohji Yamamoto, Yutaka Banno, Yongqiang Wang, Kozo Tsuchida, Yutaka Kawaguchi, Hiroshi Fujii

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The silk gland of Bombyx mori is an organ specialized for the synthesis and secretion of silk proteins. We report here the resolution of silk gland proteins by 2-DE and the identification of many of those proteins. This was accomplished by dissecting the glands into several sections, with each exhibiting more than 400 protein spots by 2-DE, of which 100 spots were excised and characterized by in-gel digestion followed by PMF. Ninety-three proteins were tentatively identified. These were then categorized into groups involved in silk protein secretion, transport, lipid metabolism, defense, etc. Western blotting of a 2-DE gel using an antibody of the carotenoid binding protein confirmed the presence of this protein in the silk gland. Proteins including fibroin L-chain and P25 were found as multiple isoforms, some of which contained differential amounts of phosphate residues as analyzed by on-probe dephosphorylation. The current analysis contributes to our understanding of proteins expressed by the silk gland not only of the model lepidopteran B. mori, but also to proteins from other silk-producing insects such as Philosamia cynthia ricini.

Original languageEnglish
Pages (from-to)2586-2599
Number of pages14
JournalProteomics
Volume6
Issue number8
DOIs
Publication statusPublished - Apr 1 2006

Fingerprint

Bombyx
Silk
Proteome
Proteins
Gels
Fibroins
Protein Transport
Carotenoids
Lipid Metabolism
Insects
Digestion
Carrier Proteins
Protein Isoforms
Western Blotting
Phosphates
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Proteome analysis of silk gland proteins from the silkworm, Bombyx mori. / Zhang, Pingbo; Aso, Yoichi; Yamamoto, Kohji; Banno, Yutaka; Wang, Yongqiang; Tsuchida, Kozo; Kawaguchi, Yutaka; Fujii, Hiroshi.

In: Proteomics, Vol. 6, No. 8, 01.04.2006, p. 2586-2599.

Research output: Contribution to journalArticle

Zhang, P, Aso, Y, Yamamoto, K, Banno, Y, Wang, Y, Tsuchida, K, Kawaguchi, Y & Fujii, H 2006, 'Proteome analysis of silk gland proteins from the silkworm, Bombyx mori', Proteomics, vol. 6, no. 8, pp. 2586-2599. https://doi.org/10.1002/pmic.200500348
Zhang, Pingbo ; Aso, Yoichi ; Yamamoto, Kohji ; Banno, Yutaka ; Wang, Yongqiang ; Tsuchida, Kozo ; Kawaguchi, Yutaka ; Fujii, Hiroshi. / Proteome analysis of silk gland proteins from the silkworm, Bombyx mori. In: Proteomics. 2006 ; Vol. 6, No. 8. pp. 2586-2599.
@article{307e52857f0a46978e22a8c99ccf939e,
title = "Proteome analysis of silk gland proteins from the silkworm, Bombyx mori",
abstract = "The silk gland of Bombyx mori is an organ specialized for the synthesis and secretion of silk proteins. We report here the resolution of silk gland proteins by 2-DE and the identification of many of those proteins. This was accomplished by dissecting the glands into several sections, with each exhibiting more than 400 protein spots by 2-DE, of which 100 spots were excised and characterized by in-gel digestion followed by PMF. Ninety-three proteins were tentatively identified. These were then categorized into groups involved in silk protein secretion, transport, lipid metabolism, defense, etc. Western blotting of a 2-DE gel using an antibody of the carotenoid binding protein confirmed the presence of this protein in the silk gland. Proteins including fibroin L-chain and P25 were found as multiple isoforms, some of which contained differential amounts of phosphate residues as analyzed by on-probe dephosphorylation. The current analysis contributes to our understanding of proteins expressed by the silk gland not only of the model lepidopteran B. mori, but also to proteins from other silk-producing insects such as Philosamia cynthia ricini.",
author = "Pingbo Zhang and Yoichi Aso and Kohji Yamamoto and Yutaka Banno and Yongqiang Wang and Kozo Tsuchida and Yutaka Kawaguchi and Hiroshi Fujii",
year = "2006",
month = "4",
day = "1",
doi = "10.1002/pmic.200500348",
language = "English",
volume = "6",
pages = "2586--2599",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "8",

}

TY - JOUR

T1 - Proteome analysis of silk gland proteins from the silkworm, Bombyx mori

AU - Zhang, Pingbo

AU - Aso, Yoichi

AU - Yamamoto, Kohji

AU - Banno, Yutaka

AU - Wang, Yongqiang

AU - Tsuchida, Kozo

AU - Kawaguchi, Yutaka

AU - Fujii, Hiroshi

PY - 2006/4/1

Y1 - 2006/4/1

N2 - The silk gland of Bombyx mori is an organ specialized for the synthesis and secretion of silk proteins. We report here the resolution of silk gland proteins by 2-DE and the identification of many of those proteins. This was accomplished by dissecting the glands into several sections, with each exhibiting more than 400 protein spots by 2-DE, of which 100 spots were excised and characterized by in-gel digestion followed by PMF. Ninety-three proteins were tentatively identified. These were then categorized into groups involved in silk protein secretion, transport, lipid metabolism, defense, etc. Western blotting of a 2-DE gel using an antibody of the carotenoid binding protein confirmed the presence of this protein in the silk gland. Proteins including fibroin L-chain and P25 were found as multiple isoforms, some of which contained differential amounts of phosphate residues as analyzed by on-probe dephosphorylation. The current analysis contributes to our understanding of proteins expressed by the silk gland not only of the model lepidopteran B. mori, but also to proteins from other silk-producing insects such as Philosamia cynthia ricini.

AB - The silk gland of Bombyx mori is an organ specialized for the synthesis and secretion of silk proteins. We report here the resolution of silk gland proteins by 2-DE and the identification of many of those proteins. This was accomplished by dissecting the glands into several sections, with each exhibiting more than 400 protein spots by 2-DE, of which 100 spots were excised and characterized by in-gel digestion followed by PMF. Ninety-three proteins were tentatively identified. These were then categorized into groups involved in silk protein secretion, transport, lipid metabolism, defense, etc. Western blotting of a 2-DE gel using an antibody of the carotenoid binding protein confirmed the presence of this protein in the silk gland. Proteins including fibroin L-chain and P25 were found as multiple isoforms, some of which contained differential amounts of phosphate residues as analyzed by on-probe dephosphorylation. The current analysis contributes to our understanding of proteins expressed by the silk gland not only of the model lepidopteran B. mori, but also to proteins from other silk-producing insects such as Philosamia cynthia ricini.

UR - http://www.scopus.com/inward/record.url?scp=33646236906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646236906&partnerID=8YFLogxK

U2 - 10.1002/pmic.200500348

DO - 10.1002/pmic.200500348

M3 - Article

C2 - 16548058

AN - SCOPUS:33646236906

VL - 6

SP - 2586

EP - 2599

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 8

ER -