Proteomic analysis of human brain identifies α-enolase as a novel autoantigen in Hashimoto's encephalopathy

Hirofumi Ochi, Izumi Horiuchi, Norie Araki, Tosifusa Toda, Tomohiro Araki, Kaori Sato, Hiroyuki Murai, Manabu Osoegawa, Takeshi Yamada, Ken Okamura, Tomoaki Ogino, Kiyohisa Mizumoto, Hirohumi Yamashita, Hideyuki Saya, Jun-Ichi Kira

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Hashimoto's encephalopathy (HE) is a rare autoimmune disease associated with Hashimoto's thyroiditis (HT). To identify the HE-related autoantigens, we developed a human brain proteome map using two-dimensional electrophoresis and applied it to the immuno-screening of brain proteins that react with autoantibodies in HE patients. After sequential MALDI-TOF-MASS analysis, immuno-positive spots of 48 kDa (pI 7.3-7.8) detected from HE patient sera were identified as a novel autoimmuno-antigen, α-enolase, harboring several modifications. Specific high reactivities against human α-enolase were significant in HE patients with excellent corticosteroid sensitivity, whereas the patients with fair or poor sensitivity to the corticosteroid treatment showed less reactivities than cut-off level. Although a few HT patients showed faint reactions to α-enolase, 95% of HT patients, patients with other neurological disorders, and healthy subjects tested were all negative. These results suggest that the detection of anti-α-enolase antibody is useful for defining HE-related pathology, and this proteomic strategy is a powerful method for identifying autoantigens of various central nervous system diseases with unknown autoimmune etiologies.

Original languageEnglish
Pages (from-to)197-202
Number of pages6
JournalFEBS Letters
Volume528
Issue number1-3
DOIs
Publication statusPublished - Sep 25 2002

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Phosphopyruvate Hydratase
Autoantigens
Proteomics
Brain
Hashimoto Disease
Adrenal Cortex Hormones
Neurology
Pathology
Proteome
Electrophoresis
Autoantibodies
Screening
Antigens
Central Nervous System Diseases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Rare Diseases
Hashimoto's encephalitis
Antibodies
Nervous System Diseases
Autoimmune Diseases

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Proteomic analysis of human brain identifies α-enolase as a novel autoantigen in Hashimoto's encephalopathy. / Ochi, Hirofumi; Horiuchi, Izumi; Araki, Norie; Toda, Tosifusa; Araki, Tomohiro; Sato, Kaori; Murai, Hiroyuki; Osoegawa, Manabu; Yamada, Takeshi; Okamura, Ken; Ogino, Tomoaki; Mizumoto, Kiyohisa; Yamashita, Hirohumi; Saya, Hideyuki; Kira, Jun-Ichi.

In: FEBS Letters, Vol. 528, No. 1-3, 25.09.2002, p. 197-202.

Research output: Contribution to journalArticle

Ochi, H, Horiuchi, I, Araki, N, Toda, T, Araki, T, Sato, K, Murai, H, Osoegawa, M, Yamada, T, Okamura, K, Ogino, T, Mizumoto, K, Yamashita, H, Saya, H & Kira, J-I 2002, 'Proteomic analysis of human brain identifies α-enolase as a novel autoantigen in Hashimoto's encephalopathy', FEBS Letters, vol. 528, no. 1-3, pp. 197-202. https://doi.org/10.1016/S0014-5793(02)03307-0
Ochi, Hirofumi ; Horiuchi, Izumi ; Araki, Norie ; Toda, Tosifusa ; Araki, Tomohiro ; Sato, Kaori ; Murai, Hiroyuki ; Osoegawa, Manabu ; Yamada, Takeshi ; Okamura, Ken ; Ogino, Tomoaki ; Mizumoto, Kiyohisa ; Yamashita, Hirohumi ; Saya, Hideyuki ; Kira, Jun-Ichi. / Proteomic analysis of human brain identifies α-enolase as a novel autoantigen in Hashimoto's encephalopathy. In: FEBS Letters. 2002 ; Vol. 528, No. 1-3. pp. 197-202.
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