Proteomic analysis of in vivo 14-3-3 interactions in the yeast Saccharomyces cerevisiae

Kazue Kakiuchi, Yoshio Yamauchi, Masato Taoka, Maki Iwago, Tomoko Fujita, Takashi Ito, Si Young Song, Akira Sakai, Toshiaki Isobe, Tohru Ichimura

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

The yeast Saccharomyces cerevisiae produces two 14-3-3 proteins, Bmh1 and Bmh2, whose exact functions have remained unclear. Here, we performed a comprehensive proteomic analysis using multistep immunoaffinity purification and mass spectrometry and identified 271 yeast proteins that specifically bind to Bmh1 and -2 in a phosphorylation-dependent manner. The identified proteins have diverse biochemical functions and cellular roles, including cell signaling, metabolism, and cell cycle regulation. Importantly, there are a number of protein subsets that are involved in the regulation of yeast physiology through a variety of cell signaling pathways, including stress-induced transcription, cell division, and chitin synthesis at the cell wall. In fact, we found that a yeast mutant deficient in Bmh1 and -2 had defects in signal-dependent response of the MAPK (Hog1 and Mpk1) cascade and exhibited an abnormal accumulation of chitin at the bud neck. We propose that Bmh1 and -2 are common regulators of many cell signaling modules and pathways mediated by protein phosphorylation and regulate a variety of biological events by coordinately controlling the identified multiplex phosphoprotein components.

Original languageEnglish
Pages (from-to)7781-7792
Number of pages12
JournalBiochemistry
Volume46
Issue number26
DOIs
Publication statusPublished - Jul 3 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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