Proteomic and metabolomic analyses of the white-rot fungus phanerochaete chrysosporium exposed to exogenous benzoic acid

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Intracellular processes of the white-rot basidiomycete Phanerochaete chrysosporium involved in the metabolism of benzoic acid (BA) were investigated at the proteome and metabolome level. Up-regulation of aryl-alcohol dehydrogenase, arylaldehyde dehydrogenase, and cytochrome P450s was observed upon addition of exogenous BA, suggesting that these enzymes play key roles in its metabolism. Intracellular metabolic shifts from the short-cut TCA/glyoxylate bicycle system to the TCA cycle and an increased flux in the TCA cycle indicated activation of the heme biosynthetic pathway and the production of NAD(P)H. In addition, combined analyses of proteome and metabolome clearly indicated the role of trehalose as a storage disaccharide and that the mannitol cycle plays a role in an alternative energy-producing pathway.

Original languageEnglish
Pages (from-to)2342-2350
Number of pages9
JournalJournal of Proteome Research
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 1 2008

Fingerprint

Phanerochaete
Benzoic Acid
Metabolomics
Metabolome
Proteome
Fungi
Metabolism
Proteomics
Basidiomycota
Trehalose
Bicycles
Disaccharides
Biosynthetic Pathways
Mannitol
Cytochromes
Heme
NAD
Oxidoreductases
Up-Regulation
Chemical activation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

@article{0541f29773684a599e55f32c1a728369,
title = "Proteomic and metabolomic analyses of the white-rot fungus phanerochaete chrysosporium exposed to exogenous benzoic acid",
abstract = "Intracellular processes of the white-rot basidiomycete Phanerochaete chrysosporium involved in the metabolism of benzoic acid (BA) were investigated at the proteome and metabolome level. Up-regulation of aryl-alcohol dehydrogenase, arylaldehyde dehydrogenase, and cytochrome P450s was observed upon addition of exogenous BA, suggesting that these enzymes play key roles in its metabolism. Intracellular metabolic shifts from the short-cut TCA/glyoxylate bicycle system to the TCA cycle and an increased flux in the TCA cycle indicated activation of the heme biosynthetic pathway and the production of NAD(P)H. In addition, combined analyses of proteome and metabolome clearly indicated the role of trehalose as a storage disaccharide and that the mannitol cycle plays a role in an alternative energy-producing pathway.",
author = "Fumiko Matsuzaki and Motoyuki Shimizu and Hiroyuki Wariishi",
year = "2008",
month = "6",
day = "1",
doi = "10.1021/pr700617s",
language = "English",
volume = "7",
pages = "2342--2350",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Proteomic and metabolomic analyses of the white-rot fungus phanerochaete chrysosporium exposed to exogenous benzoic acid

AU - Matsuzaki, Fumiko

AU - Shimizu, Motoyuki

AU - Wariishi, Hiroyuki

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Intracellular processes of the white-rot basidiomycete Phanerochaete chrysosporium involved in the metabolism of benzoic acid (BA) were investigated at the proteome and metabolome level. Up-regulation of aryl-alcohol dehydrogenase, arylaldehyde dehydrogenase, and cytochrome P450s was observed upon addition of exogenous BA, suggesting that these enzymes play key roles in its metabolism. Intracellular metabolic shifts from the short-cut TCA/glyoxylate bicycle system to the TCA cycle and an increased flux in the TCA cycle indicated activation of the heme biosynthetic pathway and the production of NAD(P)H. In addition, combined analyses of proteome and metabolome clearly indicated the role of trehalose as a storage disaccharide and that the mannitol cycle plays a role in an alternative energy-producing pathway.

AB - Intracellular processes of the white-rot basidiomycete Phanerochaete chrysosporium involved in the metabolism of benzoic acid (BA) were investigated at the proteome and metabolome level. Up-regulation of aryl-alcohol dehydrogenase, arylaldehyde dehydrogenase, and cytochrome P450s was observed upon addition of exogenous BA, suggesting that these enzymes play key roles in its metabolism. Intracellular metabolic shifts from the short-cut TCA/glyoxylate bicycle system to the TCA cycle and an increased flux in the TCA cycle indicated activation of the heme biosynthetic pathway and the production of NAD(P)H. In addition, combined analyses of proteome and metabolome clearly indicated the role of trehalose as a storage disaccharide and that the mannitol cycle plays a role in an alternative energy-producing pathway.

UR - http://www.scopus.com/inward/record.url?scp=49849100967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49849100967&partnerID=8YFLogxK

U2 - 10.1021/pr700617s

DO - 10.1021/pr700617s

M3 - Article

C2 - 18435559

AN - SCOPUS:49849100967

VL - 7

SP - 2342

EP - 2350

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 6

ER -