Proteomic and metabolomic analyses of the white-rot fungus phanerochaete chrysosporium exposed to exogenous benzoic acid

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Abstract

Intracellular processes of the white-rot basidiomycete Phanerochaete chrysosporium involved in the metabolism of benzoic acid (BA) were investigated at the proteome and metabolome level. Up-regulation of aryl-alcohol dehydrogenase, arylaldehyde dehydrogenase, and cytochrome P450s was observed upon addition of exogenous BA, suggesting that these enzymes play key roles in its metabolism. Intracellular metabolic shifts from the short-cut TCA/glyoxylate bicycle system to the TCA cycle and an increased flux in the TCA cycle indicated activation of the heme biosynthetic pathway and the production of NAD(P)H. In addition, combined analyses of proteome and metabolome clearly indicated the role of trehalose as a storage disaccharide and that the mannitol cycle plays a role in an alternative energy-producing pathway.

Original languageEnglish
Pages (from-to)2342-2350
Number of pages9
JournalJournal of Proteome Research
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 1 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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