Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis

Pingbo Zhang, Yoichi Aso, Hiroyuki Jikuya, Takahiro Kusakabe, Man Lee, Yutaka Kawaguchi, Kohji Yamamoto, Yutaka Banno, Hiroshi Fujii

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.

Original languageEnglish
Pages (from-to)2295-2303
Number of pages9
JournalJournal of Proteome Research
Volume6
Issue number6
DOIs
Publication statusPublished - Jun 1 2007

All Science Journal Classification (ASJC) codes

  • Genetics
  • Biotechnology
  • Biochemistry

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