Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis

Pingbo Zhang, Yoichi Aso, Hiroyuki Jikuya, Takahiro Kusakabe, Man Lee, Yutaka Kawaguchi, Kohji Yamamoto, Yutaka Banno, Hiroshi Fujii

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.

Original languageEnglish
Pages (from-to)2295-2303
Number of pages9
JournalJournal of Proteome Research
Volume6
Issue number6
DOIs
Publication statusPublished - Jun 1 2007

Fingerprint

Bombyx
Muscle Proteins
Proteomics
Skeletal Muscle
Pupa
Proteins
Larva
Insects
Signal transduction
Biochemistry
Troponin I
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Immunoblotting
Metabolism
Muscle
Signal Transduction
Protein Isoforms
Gels
Muscles

All Science Journal Classification (ASJC) codes

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis. / Zhang, Pingbo; Aso, Yoichi; Jikuya, Hiroyuki; Kusakabe, Takahiro; Lee, Man; Kawaguchi, Yutaka; Yamamoto, Kohji; Banno, Yutaka; Fujii, Hiroshi.

In: Journal of Proteome Research, Vol. 6, No. 6, 01.06.2007, p. 2295-2303.

Research output: Contribution to journalArticle

Zhang, Pingbo ; Aso, Yoichi ; Jikuya, Hiroyuki ; Kusakabe, Takahiro ; Lee, Man ; Kawaguchi, Yutaka ; Yamamoto, Kohji ; Banno, Yutaka ; Fujii, Hiroshi. / Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis. In: Journal of Proteome Research. 2007 ; Vol. 6, No. 6. pp. 2295-2303.
@article{ea0051da502648cdb004354ca0068f94,
title = "Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis",
abstract = "The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.",
author = "Pingbo Zhang and Yoichi Aso and Hiroyuki Jikuya and Takahiro Kusakabe and Man Lee and Yutaka Kawaguchi and Kohji Yamamoto and Yutaka Banno and Hiroshi Fujii",
year = "2007",
month = "6",
day = "1",
doi = "10.1021/pr070071y",
language = "English",
volume = "6",
pages = "2295--2303",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Proteomic profiling of the silkworm skeletal muscle proteins during larval-pupal metamorphosis

AU - Zhang, Pingbo

AU - Aso, Yoichi

AU - Jikuya, Hiroyuki

AU - Kusakabe, Takahiro

AU - Lee, Man

AU - Kawaguchi, Yutaka

AU - Yamamoto, Kohji

AU - Banno, Yutaka

AU - Fujii, Hiroshi

PY - 2007/6/1

Y1 - 2007/6/1

N2 - The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.

AB - The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.

UR - http://www.scopus.com/inward/record.url?scp=34250843824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250843824&partnerID=8YFLogxK

U2 - 10.1021/pr070071y

DO - 10.1021/pr070071y

M3 - Article

C2 - 17497908

AN - SCOPUS:34250843824

VL - 6

SP - 2295

EP - 2303

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 6

ER -