Proteomics finding heat shock protein 27 as a biomarker for resistance of pancreatic cancer cells to gemcitabine

Sayaka Mori-Iwamoto, Yasuhiro Kuramitsu, Shomei Ryozawa, Kuniko Mikuria, Masanori Fujimoto, Shin Ichiro Maehara, Yoshihiro Maehara, Kiwamu Okita, Kazuyuki Nakamura, Isao Sakaida

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Abstract

Pancreatic cancer remains a devastating disease and >96% of patients with pancreatic cancer do not survive for more than 5 years. Gemcitabine (2′-deoxy-2′-difluorodeoxycytidine: Gemzar) appears to be the only clinically effective drug for pancreatic cancer, but it has little impact on outcome. Proteomic analysis of gemcitabine-sensitive cells (KLM1) and resistant pancreatic cells (KLM1-R) was performed to identify target proteins of the gemcitabine. We found seven proteins, HSP27, peroxiredoxin 2, endoplasmic reticulum protein ERp29 precursor, 6-phosphogluconolactonase, triosphospate isomerase, α enolase, and nucleophosmine that could play a role in determining the sensitivity of pancreatic cancer to gemcitabine. We knocked down HSP27 in KLM1-R and the sensitivity to gemcitabine was restored. In addition, increased HSP27 expression in tumor specimens was related to higher resistibility to gemcitabine in patients of pancreatic cancer. HSP27 may play an important role in the resistibility to gemcitabine, and it could also be a possible biomarker for predicting the response of pancreatic cancer patients to treatment with gemcitabine.

Original languageEnglish
Pages (from-to)1345-1350
Number of pages6
JournalInternational journal of oncology
Volume31
Issue number6
Publication statusPublished - Dec 1 2007

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Mori-Iwamoto, S., Kuramitsu, Y., Ryozawa, S., Mikuria, K., Fujimoto, M., Maehara, S. I., ... Sakaida, I. (2007). Proteomics finding heat shock protein 27 as a biomarker for resistance of pancreatic cancer cells to gemcitabine. International journal of oncology, 31(6), 1345-1350.