Abstract
Prothymosin alpha (PT-α) is a nuclear protein involved in cell proliferation. Transcription of PT-a has been reported to be regulated by the c-myc gene in vitro. We identified PT-x as being overexpressed in a human colon cancer minus normal mucosa subtraction cDNA library. Northern blot (messenger RNA) analysis showed that both PT-α and c-myc genes were overexpressed in human colorectal cancers compared with adjacent normal tissues. Immunohistochemical studies for PT-α and c-myc supported these findings. There was no correlation between PT-α or c-myc messenger RNA expression and Dukes' stage of colorectal cancer; or between either of these two and actin messenger RNA expression. There was, however, a significant correlation between the PT-α expression and c-myc expression (P < 0.001). These findings support the hypothesis that PT-α gene transcription may be associated with, and possibly under the control of, the c-myc gene in human colorectal cancers.
| Original language | English |
|---|---|
| Pages (from-to) | 2821-2826 |
| Number of pages | 6 |
| Journal | Oncogene |
| Volume | 8 |
| Issue number | 10 |
| Publication status | Published - Jan 1 1993 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
- Cancer Research
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Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer. / Mori, M.; Barnard, G. F.; Staniunas, R. J.; Jessup, J. M.; Steele, G. D.; Chen, L. B.
In: Oncogene, Vol. 8, No. 10, 01.01.1993, p. 2821-2826.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer
AU - Mori, M.
AU - Barnard, G. F.
AU - Staniunas, R. J.
AU - Jessup, J. M.
AU - Steele, G. D.
AU - Chen, L. B.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Prothymosin alpha (PT-α) is a nuclear protein involved in cell proliferation. Transcription of PT-a has been reported to be regulated by the c-myc gene in vitro. We identified PT-x as being overexpressed in a human colon cancer minus normal mucosa subtraction cDNA library. Northern blot (messenger RNA) analysis showed that both PT-α and c-myc genes were overexpressed in human colorectal cancers compared with adjacent normal tissues. Immunohistochemical studies for PT-α and c-myc supported these findings. There was no correlation between PT-α or c-myc messenger RNA expression and Dukes' stage of colorectal cancer; or between either of these two and actin messenger RNA expression. There was, however, a significant correlation between the PT-α expression and c-myc expression (P < 0.001). These findings support the hypothesis that PT-α gene transcription may be associated with, and possibly under the control of, the c-myc gene in human colorectal cancers.
AB - Prothymosin alpha (PT-α) is a nuclear protein involved in cell proliferation. Transcription of PT-a has been reported to be regulated by the c-myc gene in vitro. We identified PT-x as being overexpressed in a human colon cancer minus normal mucosa subtraction cDNA library. Northern blot (messenger RNA) analysis showed that both PT-α and c-myc genes were overexpressed in human colorectal cancers compared with adjacent normal tissues. Immunohistochemical studies for PT-α and c-myc supported these findings. There was no correlation between PT-α or c-myc messenger RNA expression and Dukes' stage of colorectal cancer; or between either of these two and actin messenger RNA expression. There was, however, a significant correlation between the PT-α expression and c-myc expression (P < 0.001). These findings support the hypothesis that PT-α gene transcription may be associated with, and possibly under the control of, the c-myc gene in human colorectal cancers.
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M3 - Article
C2 - 8378090
AN - SCOPUS:0027172019
VL - 8
SP - 2821
EP - 2826
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 10
ER -