Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer

M. Mori, G. F. Barnard, R. J. Staniunas, J. M. Jessup, G. D. Steele, L. B. Chen

Research output: Contribution to journalArticle

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Abstract

Prothymosin alpha (PT-α) is a nuclear protein involved in cell proliferation. Transcription of PT-a has been reported to be regulated by the c-myc gene in vitro. We identified PT-x as being overexpressed in a human colon cancer minus normal mucosa subtraction cDNA library. Northern blot (messenger RNA) analysis showed that both PT-α and c-myc genes were overexpressed in human colorectal cancers compared with adjacent normal tissues. Immunohistochemical studies for PT-α and c-myc supported these findings. There was no correlation between PT-α or c-myc messenger RNA expression and Dukes' stage of colorectal cancer; or between either of these two and actin messenger RNA expression. There was, however, a significant correlation between the PT-α expression and c-myc expression (P < 0.001). These findings support the hypothesis that PT-α gene transcription may be associated with, and possibly under the control of, the c-myc gene in human colorectal cancers.

Original languageEnglish
Pages (from-to)2821-2826
Number of pages6
JournalOncogene
Volume8
Issue number10
Publication statusPublished - Jan 1 1993
Externally publishedYes

Fingerprint

myc Genes
Colonic Neoplasms
Colorectal Neoplasms
Messenger RNA
Nuclear Proteins
Gene Library
Northern Blotting
Actins
Mucous Membrane
Cell Proliferation
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Mori, M., Barnard, G. F., Staniunas, R. J., Jessup, J. M., Steele, G. D., & Chen, L. B. (1993). Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer. Oncogene, 8(10), 2821-2826.

Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer. / Mori, M.; Barnard, G. F.; Staniunas, R. J.; Jessup, J. M.; Steele, G. D.; Chen, L. B.

In: Oncogene, Vol. 8, No. 10, 01.01.1993, p. 2821-2826.

Research output: Contribution to journalArticle

Mori, M, Barnard, GF, Staniunas, RJ, Jessup, JM, Steele, GD & Chen, LB 1993, 'Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer', Oncogene, vol. 8, no. 10, pp. 2821-2826.
Mori M, Barnard GF, Staniunas RJ, Jessup JM, Steele GD, Chen LB. Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer. Oncogene. 1993 Jan 1;8(10):2821-2826.
Mori, M. ; Barnard, G. F. ; Staniunas, R. J. ; Jessup, J. M. ; Steele, G. D. ; Chen, L. B. / Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer. In: Oncogene. 1993 ; Vol. 8, No. 10. pp. 2821-2826.
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