Protocol for high-resolution separation of rodent myosin heavy chain isoforms in a mini-gel electrophoresis system

Wataru Mizunoya, Jun ichiro Wakamatsu, Ryuichi Tatsumi, Yoshihide Ikeuchi

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Skeletal muscle comprises several fiber types classified based on their contractile and metabolic properties. Skeletal muscle fiber types are classified according to their myosin heavy chain isoforms (MyHC I, IIa, IIx, and IIb). We attained good separation of MyHC isoforms in a mini-gel system by modifying a previously developed electrophoresis protocol. Increased glycerol and decreased cross-linking agent concentrations improved the separation of MyHC isoforms. Sample preparation with dithiothreitol and protease inhibitors produced clear MyHC band boundaries. This protocol included silver staining, with a linear range. The protocol provided high resolution and a highly accurate assay of rodent MyHC isoforms.

Original languageEnglish
Pages (from-to)111-113
Number of pages3
JournalAnalytical Biochemistry
Volume377
Issue number1
DOIs
Publication statusPublished - Jun 1 2008

Fingerprint

Myosin Heavy Chains
Electrophoresis
Rodentia
Protein Isoforms
Gels
Muscle
Silver Staining
Fibers
Dithiothreitol
Skeletal Muscle Fibers
Protease Inhibitors
Silver
Glycerol
Assays
Skeletal Muscle

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Protocol for high-resolution separation of rodent myosin heavy chain isoforms in a mini-gel electrophoresis system. / Mizunoya, Wataru; Wakamatsu, Jun ichiro; Tatsumi, Ryuichi; Ikeuchi, Yoshihide.

In: Analytical Biochemistry, Vol. 377, No. 1, 01.06.2008, p. 111-113.

Research output: Contribution to journalArticle

Mizunoya, Wataru ; Wakamatsu, Jun ichiro ; Tatsumi, Ryuichi ; Ikeuchi, Yoshihide. / Protocol for high-resolution separation of rodent myosin heavy chain isoforms in a mini-gel electrophoresis system. In: Analytical Biochemistry. 2008 ; Vol. 377, No. 1. pp. 111-113.
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