Protocol for rat single muscle fiber isolation and culture

Yusuke Komiya, Judy E. Anderson, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To attain a superior in vitro model of mature muscle fibers, we modified the established protocol for isolating single muscle fibers from rat skeletal muscle. Muscle fiber cultures with high viability were obtained using flexor digitorum brevis muscle and lasted for at least 7 days. We compared the expression levels of adult myosin heavy chain (MyHC) isoforms in these single muscle fibers with myotubes formed from myoblasts; isolated fibers contained markedly more abundant adult MyHC isoforms than myotubes. This muscle fiber model, therefore, will be useful for studying the various functions and cellular processes of mature muscles in vitro.

Original languageEnglish
Pages (from-to)22-24
Number of pages3
JournalAnalytical Biochemistry
Volume482
DOIs
Publication statusPublished - Jun 1 2015

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Muscle
Rats
Skeletal Muscle Fibers
Muscles
Fibers
Myosin Heavy Chains
Protein Isoforms
Myoblasts
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Protocol for rat single muscle fiber isolation and culture. / Komiya, Yusuke; Anderson, Judy E.; Akahoshi, Mariko; Nakamura, Mako; Tatsumi, Ryuichi; Ikeuchi, Yoshihide; Mizunoya, Wataru.

In: Analytical Biochemistry, Vol. 482, 01.06.2015, p. 22-24.

Research output: Contribution to journalArticle

Komiya, Yusuke ; Anderson, Judy E. ; Akahoshi, Mariko ; Nakamura, Mako ; Tatsumi, Ryuichi ; Ikeuchi, Yoshihide ; Mizunoya, Wataru. / Protocol for rat single muscle fiber isolation and culture. In: Analytical Biochemistry. 2015 ; Vol. 482. pp. 22-24.
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