Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

Soichiro Sonoda; Haruyoshi Yamaza; Koichiro Yoshimaru; Tomoaki Taguchi; Takayoshi Yamaza

doi:10.1016/j.xpro.2022.101386

Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

Soichiro Sonoda, Haruyoshi Yamaza, Koichiro Yoshimaru, Tomoaki Taguchi, Takayoshi Yamaza

Research output: Contribution to journal › Article › peer-review

Abstract

Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).

Original language	English
Article number	101386
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101386
Publication status	Published - Jun 17 2022

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Neuroscience(all)
Immunology and Microbiology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2022.101386

Cite this

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title = "Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells",

abstract = "Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).",

author = "Soichiro Sonoda and Haruyoshi Yamaza and Koichiro Yoshimaru and Tomoaki Taguchi and Takayoshi Yamaza",

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AU - Yoshimaru, Koichiro

AU - Taguchi, Tomoaki

AU - Yamaza, Takayoshi

N1 - Funding Information: We thank all the authors of the original study ( Iwanaka et al., 2020 ) from which this protocol was generated. This work was supported by the grants-in-aid for Early-Career Scientists (JSPS KAKENHI grant number JP19K18945 and JP21K16932 to S.S.) of Japan Society for the Promotion of Science (JSPS). Publisher Copyright: © 2022

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N2 - Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).

AB - Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).

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Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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