Protogenin, a new member of the immunoglobulin superfamily, is implicated in the development of the mouse lower first molar

Keiko F. Takahashi, Tamotsu Kiyoshima, Ieyoshi Kobayashi, Ming Xie, Haruyoshi Yamaza, Hiroaki Fujiwara, Yukiko Ookuma, Kengo Nagata, Hiroko Wada, Takako Sakai, Yoshihiro Terada, Hidetaka Sakai

Research output: Contribution to journalArticle

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Abstract

Background. Protogenin (Prtg) has been identified as a gene which is highly expressed in the mouse mandible at embryonic day 10.5 (E10.5) by a cDNA subtraction method between mandibles at E10.5 and E12.0. Prtg is a new member of the deleted in colorectal carcinoma (DCC) family, which is composed of DCC, Neogenin, Punc and Nope. Although these members play an important role in the development of the embryonic central nervous system, recent research has also shed on the non-neuronal organization. However, very little is known regarding the fetal requirement of the non-neuronal organization for Prtg and how this may be associated with the tooth germ development. This study examined the functional implications of Prtg in the developing tooth germ of the mouse lower first molar. Results. Ptrg is preferentially expressed in the early stage of organogenesis. Prtg mRNA and protein were widely expressed in the mesenchymal cells in the mandible at E10.5. The oral epithelial cells were also positive for Prtg. The expression intensity of Prtg after E12.0 was markedly reduced in the mesenchymal cells of the mandible, and was restricted to the area where the tooth bud was likely to be formed. Signals were also observed in the epithelial cells of the tooth germ. Weak signals were observed in the inner enamel epithelial cells at E16.0 and E18.0. An inhibition assay using a hemagglutinating virus of Japan-liposome containing Prtg antisense- phosphorothioated-oligodeoxynucleotide (AS-S-ODN) in cultured mandibles at E10.5 showed a significant growth inhibition in the tooth germ. The relationship between Prtg and the odontogenesis-related genes was examined in mouse E10.5 mandible, and we verified that the Bmp-4 expression had significantly been decreased in the mouse E10.5 mandible 24 hr after treatment with Prtg AS-S-ODN. Conclusion. These results indicated that the Prtg might be related to the initial morphogenesis of the tooth germ leading to the differentiation of the inner enamel epithelial cells in the mouse lower first molar. A better understanding of the Prtg function might thus play a critical role in revealing a precious mechanism in tooth germ development.

Original languageEnglish
Article number115
JournalBMC Developmental Biology
Volume10
DOIs
Publication statusPublished - Nov 29 2010

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Tooth Germ
Mandible
Immunoglobulins
Epithelial Cells
Oligodeoxyribonucleotides
Dental Enamel
Colorectal Neoplasms
Odontogenesis
Sendai virus
Organogenesis
Morphogenesis
Liposomes
Genes
Embryonic Development
Tooth
Central Nervous System
Complementary DNA
Messenger RNA
Growth
Research

All Science Journal Classification (ASJC) codes

  • Developmental Biology

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Protogenin, a new member of the immunoglobulin superfamily, is implicated in the development of the mouse lower first molar. / Takahashi, Keiko F.; Kiyoshima, Tamotsu; Kobayashi, Ieyoshi; Xie, Ming; Yamaza, Haruyoshi; Fujiwara, Hiroaki; Ookuma, Yukiko; Nagata, Kengo; Wada, Hiroko; Sakai, Takako; Terada, Yoshihiro; Sakai, Hidetaka.

In: BMC Developmental Biology, Vol. 10, 115, 29.11.2010.

Research output: Contribution to journalArticle

Takahashi, Keiko F. ; Kiyoshima, Tamotsu ; Kobayashi, Ieyoshi ; Xie, Ming ; Yamaza, Haruyoshi ; Fujiwara, Hiroaki ; Ookuma, Yukiko ; Nagata, Kengo ; Wada, Hiroko ; Sakai, Takako ; Terada, Yoshihiro ; Sakai, Hidetaka. / Protogenin, a new member of the immunoglobulin superfamily, is implicated in the development of the mouse lower first molar. In: BMC Developmental Biology. 2010 ; Vol. 10.
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abstract = "Background. Protogenin (Prtg) has been identified as a gene which is highly expressed in the mouse mandible at embryonic day 10.5 (E10.5) by a cDNA subtraction method between mandibles at E10.5 and E12.0. Prtg is a new member of the deleted in colorectal carcinoma (DCC) family, which is composed of DCC, Neogenin, Punc and Nope. Although these members play an important role in the development of the embryonic central nervous system, recent research has also shed on the non-neuronal organization. However, very little is known regarding the fetal requirement of the non-neuronal organization for Prtg and how this may be associated with the tooth germ development. This study examined the functional implications of Prtg in the developing tooth germ of the mouse lower first molar. Results. Ptrg is preferentially expressed in the early stage of organogenesis. Prtg mRNA and protein were widely expressed in the mesenchymal cells in the mandible at E10.5. The oral epithelial cells were also positive for Prtg. The expression intensity of Prtg after E12.0 was markedly reduced in the mesenchymal cells of the mandible, and was restricted to the area where the tooth bud was likely to be formed. Signals were also observed in the epithelial cells of the tooth germ. Weak signals were observed in the inner enamel epithelial cells at E16.0 and E18.0. An inhibition assay using a hemagglutinating virus of Japan-liposome containing Prtg antisense- phosphorothioated-oligodeoxynucleotide (AS-S-ODN) in cultured mandibles at E10.5 showed a significant growth inhibition in the tooth germ. The relationship between Prtg and the odontogenesis-related genes was examined in mouse E10.5 mandible, and we verified that the Bmp-4 expression had significantly been decreased in the mouse E10.5 mandible 24 hr after treatment with Prtg AS-S-ODN. Conclusion. These results indicated that the Prtg might be related to the initial morphogenesis of the tooth germ leading to the differentiation of the inner enamel epithelial cells in the mouse lower first molar. A better understanding of the Prtg function might thus play a critical role in revealing a precious mechanism in tooth germ development.",
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AU - Kiyoshima, Tamotsu

AU - Kobayashi, Ieyoshi

AU - Xie, Ming

AU - Yamaza, Haruyoshi

AU - Fujiwara, Hiroaki

AU - Ookuma, Yukiko

AU - Nagata, Kengo

AU - Wada, Hiroko

AU - Sakai, Takako

AU - Terada, Yoshihiro

AU - Sakai, Hidetaka

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