Proton transport through the influenza A M2 channel

Three-dimensional reference interaction site model study

Saree Phongphanphanee, Thanyada Rungrotmongkol, Norio Yoshida, Supot Hannongbua, Fumio Hirata

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The three-dimensional distribution function (DF) and the potential of mean force (PMF) of water and hydronium ions in five protonated states of the influenza A M2 channel are calculated by means of the three-dimensional reference interaction site model (3D-RISM) theory in order to clarify the proton conduction mechanism of the channel. Each protonated state, denoted as iH, where i = 0-4, has a different number of protonated histidines, from 0 to 4. The DF of water in each state exhibits closed structures of 0H, 1H, and 2H and open structures in 3H and 4H. In the closed form, the DF and PMF indicate that hydronium ions are excluded from the channel. In contrast, the ion can distribute throughout the opened channel. The barrier in PMF of 3H, ∼3-5 kJ/mol, is lower than that of 4H, 5-7 kJ/mol, indicating that 3H has higher permeability to protons. On the basis of the radial DFs of water and hydronium ions around the imidazole rings of His37, we propose a new mechanism of proton transfer through the gating region of the channel. In this process, a hydronium ion hands a proton to a non-protonated histidine through a hydrogen bond between them, and then the other protonated histidine releases a proton to a water molecule via a hydrogen bond. The process transfers a proton effectively from one water molecule to another.

Original languageEnglish
Pages (from-to)9782-9788
Number of pages7
JournalJournal of the American Chemical Society
Volume132
Issue number28
DOIs
Publication statusPublished - Jul 21 2010
Externally publishedYes

Fingerprint

Human Influenza
Protons
Histidine
Water
Ions
Distribution functions
Hydrogen bonds
Hydrogen
Molecules
Proton transfer
Ion Channels
Permeability
Hand
hydronium ion

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Proton transport through the influenza A M2 channel : Three-dimensional reference interaction site model study. / Phongphanphanee, Saree; Rungrotmongkol, Thanyada; Yoshida, Norio; Hannongbua, Supot; Hirata, Fumio.

In: Journal of the American Chemical Society, Vol. 132, No. 28, 21.07.2010, p. 9782-9788.

Research output: Contribution to journalArticle

Phongphanphanee, Saree ; Rungrotmongkol, Thanyada ; Yoshida, Norio ; Hannongbua, Supot ; Hirata, Fumio. / Proton transport through the influenza A M2 channel : Three-dimensional reference interaction site model study. In: Journal of the American Chemical Society. 2010 ; Vol. 132, No. 28. pp. 9782-9788.
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