Provision of continuous maturation signaling to dendritic cells by RIG-I-stimulating cytosolic RNA synthesis of Sendai virus

Shinji Okano, Yoshikazu Yonemitsu, Ken Shirabe, Yoshihiro Kakeji, Yoshihiko Maehara, Mamoru Harada, Yasunobu Yoshikai, Makoto Inoue, Mamoru Hasegawa, Katsuo Sueishi

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13 Citations (Scopus)

Abstract

Dendritic cell (DC)-based immunotherapy has potential for treating infections and malignant tumors, but the functional capacity of DC must be assessed in detail, especially maturation and Ag-specific CTL priming. Recent reports suggest that DC that are provided with continuous maturation signals in vivo after transfer into patients are required to elicit the full DC functions.We demonstrate in this study that the rSendai virus vector (SeV) is a novel and ideal stimulant, providing DC with a continuous maturation signal via viral RNA synthesis in the cytosol, resulting in full maturation of monocyte-derived DC(s). Both RIG-I-dependent cytokine production and CD4 T cell responses to SeV-derived helper Ags are indispensable for overcoming regulatory T cell suppression to prime melanoma Ag recognized by T cell-1-specific CTL in the regulatory T cell abundant setting. DC stimulated via cytokine receptors, or TLRs, do not show these functional features. Therefore, SeV-infected DC have the potential for DC-directed immunotherapy.

Original languageEnglish
Pages (from-to)1828-1839
Number of pages12
JournalJournal of Immunology
Volume186
Issue number3
DOIs
Publication statusPublished - Feb 1 2011

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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