PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis

Tomoko Hirano, Hiroki Konno, Seiji Takeda, Liam Dolan, Mariko Kato, Takashi Aoyama, Takumi Higaki, Hisako Takigawa-Imamura, Masa H. Sato

Research output: Contribution to journalLetter

5 Citations (Scopus)

Abstract

Root hairs elongate by tip growth and simultaneously harden the shank by constructing the inner secondary cell wall layer. While much is known about the process of tip growth1, almost nothing is known about the mechanism by which root hairs harden the shank. Here we show that phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2), the enzymatic product of FORMATION OF APLOID AND BINUCLEATE CELLS 1 (FAB1), is involved in the hardening of the shank in root hairs in Arabidopsis. FAB1 and PtdIns(3,5)P2 localize to the plasma membrane along the shank of growing root hairs. By contrast, phosphatidylinositol 4-phosphate 5-kinase 3 (PIP5K3) and PtdIns(4,5)P2 localize to the apex of the root hair where they are required for tip growth. Reduction of FAB1 function results in the formation of wavy root hairs while those of the wild type are straight. The localization of FAB1 in the plasma membrane of the root hair shank requires the activity of Rho-related GTPases from plants 10 (ROP10) and localization of ROP10 requires FAB1 activity. Computational modelling of root hair morphogenesis successfully reproduces the wavy root hair phenotype. Taken together, these data demonstrate that root hair shank hardening requires PtdIns(3,5)P2/ROP10 signalling.

Original languageEnglish
Pages (from-to)888-897
Number of pages10
JournalNature Plants
Volume4
Issue number11
DOIs
Publication statusPublished - Nov 1 2018

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root hairs
Arabidopsis
guanosinetriphosphatase
phosphatidylinositols
cells
plasma membrane
morphogenesis
phosphotransferases (kinases)
cell walls
phosphates
phenotype

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Hirano, T., Konno, H., Takeda, S., Dolan, L., Kato, M., Aoyama, T., ... Sato, M. H. (2018). PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis. Nature Plants, 4(11), 888-897. https://doi.org/10.1038/s41477-018-0277-8

PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis. / Hirano, Tomoko; Konno, Hiroki; Takeda, Seiji; Dolan, Liam; Kato, Mariko; Aoyama, Takashi; Higaki, Takumi; Takigawa-Imamura, Hisako; Sato, Masa H.

In: Nature Plants, Vol. 4, No. 11, 01.11.2018, p. 888-897.

Research output: Contribution to journalLetter

Hirano, T, Konno, H, Takeda, S, Dolan, L, Kato, M, Aoyama, T, Higaki, T, Takigawa-Imamura, H & Sato, MH 2018, 'PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis', Nature Plants, vol. 4, no. 11, pp. 888-897. https://doi.org/10.1038/s41477-018-0277-8
Hirano T, Konno H, Takeda S, Dolan L, Kato M, Aoyama T et al. PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis. Nature Plants. 2018 Nov 1;4(11):888-897. https://doi.org/10.1038/s41477-018-0277-8
Hirano, Tomoko ; Konno, Hiroki ; Takeda, Seiji ; Dolan, Liam ; Kato, Mariko ; Aoyama, Takashi ; Higaki, Takumi ; Takigawa-Imamura, Hisako ; Sato, Masa H. / PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis. In: Nature Plants. 2018 ; Vol. 4, No. 11. pp. 888-897.
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