Abstract
Root hairs elongate by tip growth and simultaneously harden the shank by constructing the inner secondary cell wall layer. While much is known about the process of tip growth1, almost nothing is known about the mechanism by which root hairs harden the shank. Here we show that phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2), the enzymatic product of FORMATION OF APLOID AND BINUCLEATE CELLS 1 (FAB1), is involved in the hardening of the shank in root hairs in Arabidopsis. FAB1 and PtdIns(3,5)P2 localize to the plasma membrane along the shank of growing root hairs. By contrast, phosphatidylinositol 4-phosphate 5-kinase 3 (PIP5K3) and PtdIns(4,5)P2 localize to the apex of the root hair where they are required for tip growth. Reduction of FAB1 function results in the formation of wavy root hairs while those of the wild type are straight. The localization of FAB1 in the plasma membrane of the root hair shank requires the activity of Rho-related GTPases from plants 10 (ROP10) and localization of ROP10 requires FAB1 activity. Computational modelling of root hair morphogenesis successfully reproduces the wavy root hair phenotype. Taken together, these data demonstrate that root hair shank hardening requires PtdIns(3,5)P2/ROP10 signalling.
| Original language | English |
|---|---|
| Pages (from-to) | 888-897 |
| Number of pages | 10 |
| Journal | Nature Plants |
| Volume | 4 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 1 2018 |
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All Science Journal Classification (ASJC) codes
- Plant Science
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PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis. / Hirano, Tomoko; Konno, Hiroki; Takeda, Seiji; Dolan, Liam; Kato, Mariko; Aoyama, Takashi; Higaki, Takumi; Takigawa-Imamura, Hisako; Sato, Masa H.
In: Nature Plants, Vol. 4, No. 11, 01.11.2018, p. 888-897.Research output: Contribution to journal › Letter
}
TY - JOUR
T1 - PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis
AU - Hirano, Tomoko
AU - Konno, Hiroki
AU - Takeda, Seiji
AU - Dolan, Liam
AU - Kato, Mariko
AU - Aoyama, Takashi
AU - Higaki, Takumi
AU - Takigawa-Imamura, Hisako
AU - Sato, Masa H.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Root hairs elongate by tip growth and simultaneously harden the shank by constructing the inner secondary cell wall layer. While much is known about the process of tip growth1, almost nothing is known about the mechanism by which root hairs harden the shank. Here we show that phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2), the enzymatic product of FORMATION OF APLOID AND BINUCLEATE CELLS 1 (FAB1), is involved in the hardening of the shank in root hairs in Arabidopsis. FAB1 and PtdIns(3,5)P2 localize to the plasma membrane along the shank of growing root hairs. By contrast, phosphatidylinositol 4-phosphate 5-kinase 3 (PIP5K3) and PtdIns(4,5)P2 localize to the apex of the root hair where they are required for tip growth. Reduction of FAB1 function results in the formation of wavy root hairs while those of the wild type are straight. The localization of FAB1 in the plasma membrane of the root hair shank requires the activity of Rho-related GTPases from plants 10 (ROP10) and localization of ROP10 requires FAB1 activity. Computational modelling of root hair morphogenesis successfully reproduces the wavy root hair phenotype. Taken together, these data demonstrate that root hair shank hardening requires PtdIns(3,5)P2/ROP10 signalling.
AB - Root hairs elongate by tip growth and simultaneously harden the shank by constructing the inner secondary cell wall layer. While much is known about the process of tip growth1, almost nothing is known about the mechanism by which root hairs harden the shank. Here we show that phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2), the enzymatic product of FORMATION OF APLOID AND BINUCLEATE CELLS 1 (FAB1), is involved in the hardening of the shank in root hairs in Arabidopsis. FAB1 and PtdIns(3,5)P2 localize to the plasma membrane along the shank of growing root hairs. By contrast, phosphatidylinositol 4-phosphate 5-kinase 3 (PIP5K3) and PtdIns(4,5)P2 localize to the apex of the root hair where they are required for tip growth. Reduction of FAB1 function results in the formation of wavy root hairs while those of the wild type are straight. The localization of FAB1 in the plasma membrane of the root hair shank requires the activity of Rho-related GTPases from plants 10 (ROP10) and localization of ROP10 requires FAB1 activity. Computational modelling of root hair morphogenesis successfully reproduces the wavy root hair phenotype. Taken together, these data demonstrate that root hair shank hardening requires PtdIns(3,5)P2/ROP10 signalling.
UR - http://www.scopus.com/inward/record.url?scp=85055984230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055984230&partnerID=8YFLogxK
U2 - 10.1038/s41477-018-0277-8
DO - 10.1038/s41477-018-0277-8
M3 - Letter
C2 - 30390081
AN - SCOPUS:85055984230
VL - 4
SP - 888
EP - 897
JO - Nature Plants
JF - Nature Plants
SN - 2055-026X
IS - 11
ER -