TY - JOUR
T1 - PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA
AU - Ishibashi, Yohei
AU - Goda, Hatsumi
AU - Hamaguchi, Rie
AU - Sakaguchi, Keishi
AU - Sekiguchi, Takayoshi
AU - Ishiwata, Yuko
AU - Okita, Yuji
AU - Mochinaga, Seiya
AU - Ikeuchi, Shingo
AU - Mizobuchi, Takahiro
AU - Takao, Yoshitake
AU - Mori, Kazuki
AU - Tashiro, Kosuke
AU - Okino, Nozomu
AU - Honda, Daiske
AU - Hayashi, Masahiro
AU - Ito, Makoto
N1 - Funding Information:
This work was supported by The Science and Technology Research Promotion program for Agriculture, Fisheries and Food Industry, Japan (26050A). We acknowledge E. Abe, S. Kim (Kyushu University), N. Nagano, Y. Taoka, A. Matsuda (University of Miyazaki), M. Ueda and Y. Takeuchi (Konan University) for their technical assistance and valuable suggestions.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - The demand for n-3 long-chain polyunsaturated fatty acids (n-3LC-PUFAs), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), will exceed their supply in the near future, and a sustainable source of n-3LC-PUFAs is needed. Thraustochytrids are marine protists characterized by anaerobic biosynthesis of DHA via polyunsaturated fatty acid synthase (PUFA-S). Analysis of a homemade draft genome database suggested that Parietichytrium sp. lacks PUFA-S but possesses all fatty acid elongase (ELO) and desaturase (DES) genes required for DHA synthesis. The reverse genetic approach and a tracing experiment using stable isotope-labeled fatty acids revealed that the ELO/DES pathway is the only DHA synthesis pathway in Parietichytrium sp. Disruption of the C20 fatty acid ELO (C20ELO) and ∆4 fatty acid DES (∆4DES) genes with expression of ω3 fatty acid DES in this thraustochytrid allowed the production of EPA and n-3docosapentaenoic acid (n-3DPA), respectively, at the highest level among known microbial sources using fed-batch culture.
AB - The demand for n-3 long-chain polyunsaturated fatty acids (n-3LC-PUFAs), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), will exceed their supply in the near future, and a sustainable source of n-3LC-PUFAs is needed. Thraustochytrids are marine protists characterized by anaerobic biosynthesis of DHA via polyunsaturated fatty acid synthase (PUFA-S). Analysis of a homemade draft genome database suggested that Parietichytrium sp. lacks PUFA-S but possesses all fatty acid elongase (ELO) and desaturase (DES) genes required for DHA synthesis. The reverse genetic approach and a tracing experiment using stable isotope-labeled fatty acids revealed that the ELO/DES pathway is the only DHA synthesis pathway in Parietichytrium sp. Disruption of the C20 fatty acid ELO (C20ELO) and ∆4 fatty acid DES (∆4DES) genes with expression of ω3 fatty acid DES in this thraustochytrid allowed the production of EPA and n-3docosapentaenoic acid (n-3DPA), respectively, at the highest level among known microbial sources using fed-batch culture.
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U2 - 10.1038/s42003-021-02857-w
DO - 10.1038/s42003-021-02857-w
M3 - Article
C2 - 34887503
AN - SCOPUS:85121113432
VL - 4
JO - Communications Biology
JF - Communications Biology
SN - 2399-3642
IS - 1
M1 - 1378
ER -
