Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection

Yousuke Fujimoto, Shunji Hasegawa, Takeshi Matsushige, Hiroyuki Wakiguchi, Tamaki Nakamura, Hideki Hasegawa, Noriko Nakajima, Akira Ainai, Atsunori Oga, Hiroshi Itoh, Komei Shirabe, Shoichi Toda, Ryo Atsuta, Tsuneo Morishima, Shouichi Ohga

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Abstract

Asthmatic patients present more rapid progression of respiratory distress after A(H1N1)pdm09 influenza infection than after seasonal infection. Here, we sought to clarify the pathophysiology of early deterioration in asthmatic patients after A(H1N1)pdm09 infection. Cytokine levels and virus titres in bronchoalveolar lavage fluid from mice with and without asthma after A(H1N1)pdm09 or seasonal H1N1 infection were examined. In asthma/A(H1N1)pdm09 mice, IL-6 and TNF-α levels peaked at 3 days post-infection and were higher than those in all other groups. IFN-γ levels in asthma/A(H1N1)pdm09 mice at 3 days post-infection were higher than in all other mice at any time point, whereas at 7 days post-infection, the levels were lowest in asthma/A(H1N1)pdm09 mice. Virus titres in asthma/A(H1N1)pdm09 mice were highest at 3 days post-infection, and decreased by 7 days post-infection, although the levels at this time point were still higher than that in any other group. Histopathological examination showed more inflammatory cell infiltration and lung tissue destruction in the asthma/A(H1N1)pdm09 group than in any other group. The distinct cytokine profiles in A(H1N1)pdm09-infected asthmatic mice indicated excessive inflammation and virus replication within a few days after infection. Thus, bronchial asthma could be a more exacerbating factor for pandemic influenza infection than for seasonal influenza infection.

Original languageEnglish
Article number9128
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Bronchoalveolar Lavage Fluid
Human Influenza
Pneumonia
Asthma
Cytokines
Infection
Viral Load
Pandemics
Virus Replication
Inflammation

All Science Journal Classification (ASJC) codes

  • General

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Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection. / Fujimoto, Yousuke; Hasegawa, Shunji; Matsushige, Takeshi; Wakiguchi, Hiroyuki; Nakamura, Tamaki; Hasegawa, Hideki; Nakajima, Noriko; Ainai, Akira; Oga, Atsunori; Itoh, Hiroshi; Shirabe, Komei; Toda, Shoichi; Atsuta, Ryo; Morishima, Tsuneo; Ohga, Shouichi.

In: Scientific reports, Vol. 7, No. 1, 9128, 01.12.2017.

Research output: Contribution to journalArticle

Fujimoto, Y, Hasegawa, S, Matsushige, T, Wakiguchi, H, Nakamura, T, Hasegawa, H, Nakajima, N, Ainai, A, Oga, A, Itoh, H, Shirabe, K, Toda, S, Atsuta, R, Morishima, T & Ohga, S 2017, 'Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection', Scientific reports, vol. 7, no. 1, 9128. https://doi.org/10.1038/s41598-017-08030-w
Fujimoto, Yousuke ; Hasegawa, Shunji ; Matsushige, Takeshi ; Wakiguchi, Hiroyuki ; Nakamura, Tamaki ; Hasegawa, Hideki ; Nakajima, Noriko ; Ainai, Akira ; Oga, Atsunori ; Itoh, Hiroshi ; Shirabe, Komei ; Toda, Shoichi ; Atsuta, Ryo ; Morishima, Tsuneo ; Ohga, Shouichi. / Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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