Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle

Noriyo Yoshii, Yoshitoshi Ogura, Tetsuya Hayashi, Takashi Ajiro, Toshiya Sameshima, Muneo Nakazawa, Masahiro Kusumoto, Taketoshi Iwata, Masato Akiba

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.

Original languageEnglish
Pages (from-to)5719-5726
Number of pages8
JournalApplied and environmental microbiology
Volume75
Issue number17
DOIs
Publication statusPublished - Sep 1 2009
Externally publishedYes

Fingerprint

Enterohemorrhagic Escherichia coli
enterohemorrhagic Escherichia coli
Escherichia coli O157
Pulsed Field Gel Electrophoresis
pulsed-field gel electrophoresis
cattle
electrokinesis
gel
DNA Sequence Analysis
intestines
sequence analysis
genome
Genome
DNA
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle. / Yoshii, Noriyo; Ogura, Yoshitoshi; Hayashi, Tetsuya; Ajiro, Takashi; Sameshima, Toshiya; Nakazawa, Muneo; Kusumoto, Masahiro; Iwata, Taketoshi; Akiba, Masato.

In: Applied and environmental microbiology, Vol. 75, No. 17, 01.09.2009, p. 5719-5726.

Research output: Contribution to journalArticle

Yoshii, Noriyo ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Ajiro, Takashi ; Sameshima, Toshiya ; Nakazawa, Muneo ; Kusumoto, Masahiro ; Iwata, Taketoshi ; Akiba, Masato. / Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle. In: Applied and environmental microbiology. 2009 ; Vol. 75, No. 17. pp. 5719-5726.
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