Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle

Noriyo Yoshii, Yoshitoshi Ogura, Tetsuya Hayashi, Takashi Ajiro, Toshiya Sameshima, Muneo Nakazawa, Masahiro Kusumoto, Taketoshi Iwata, Masato Akiba

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.

Original languageEnglish
Pages (from-to)5719-5726
Number of pages8
JournalApplied and environmental microbiology
Volume75
Issue number17
DOIs
Publication statusPublished - Sep 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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