Abstract
A Cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of Fusarium sp. M7-1 with the formation of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. DEAE-Toyopearl 650M column chromatography showed three lyase activity peaks (fractions I, II, and III). The major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. The optimum pH was 6.5 to 8.0 and the stable pH range was 6.0 to 8.0. The purified enzyme did not degrade glucuronic or galacturonic acid-containing polysaccharides such as chondroitin, hyaluronic acid, pectin, or pectic acid. However, the purified enzyme specifically degraded various Fusarium and Gibberella acidic polysaccharides, and unsaturated sugars were produced with the release of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. These results suggest that the acidic polysaccharides derived from Fusarium and Gibberella have similar structures.
| Original language | English |
|---|---|
| Pages (from-to) | 1969-1975 |
| Number of pages | 7 |
| Journal | Agricultural and Biological Chemistry |
| Volume | 55 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Jan 1 1991 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
Cite this
Purification and Characterization of a Novel Lyase from Cellulomonas sp. that Degrades Fusarium and Gibberella Acidic Polysaccharides. / Takegawa, Kaoru; Yamaguchi, Shinya; Miki, Satoshi; Jikibara, Takayuki; Iwahara, Shojiro.
In: Agricultural and Biological Chemistry, Vol. 55, No. 8, 01.01.1991, p. 1969-1975.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification and Characterization of a Novel Lyase from Cellulomonas sp. that Degrades Fusarium and Gibberella Acidic Polysaccharides
AU - Takegawa, Kaoru
AU - Yamaguchi, Shinya
AU - Miki, Satoshi
AU - Jikibara, Takayuki
AU - Iwahara, Shojiro
PY - 1991/1/1
Y1 - 1991/1/1
N2 - A Cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of Fusarium sp. M7-1 with the formation of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. DEAE-Toyopearl 650M column chromatography showed three lyase activity peaks (fractions I, II, and III). The major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. The optimum pH was 6.5 to 8.0 and the stable pH range was 6.0 to 8.0. The purified enzyme did not degrade glucuronic or galacturonic acid-containing polysaccharides such as chondroitin, hyaluronic acid, pectin, or pectic acid. However, the purified enzyme specifically degraded various Fusarium and Gibberella acidic polysaccharides, and unsaturated sugars were produced with the release of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. These results suggest that the acidic polysaccharides derived from Fusarium and Gibberella have similar structures.
AB - A Cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of Fusarium sp. M7-1 with the formation of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. DEAE-Toyopearl 650M column chromatography showed three lyase activity peaks (fractions I, II, and III). The major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. The optimum pH was 6.5 to 8.0 and the stable pH range was 6.0 to 8.0. The purified enzyme did not degrade glucuronic or galacturonic acid-containing polysaccharides such as chondroitin, hyaluronic acid, pectin, or pectic acid. However, the purified enzyme specifically degraded various Fusarium and Gibberella acidic polysaccharides, and unsaturated sugars were produced with the release of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. These results suggest that the acidic polysaccharides derived from Fusarium and Gibberella have similar structures.
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U2 - 10.1271/bbb1961.55.1969
DO - 10.1271/bbb1961.55.1969
M3 - Article
C2 - 1368728
VL - 55
SP - 1969
EP - 1975
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 8
ER -