Purification and characterization of cytotoxin from the crude extract of Pseudomonas aeruginosa

Tetsuya Hayashi, Yoshiyuki Kamio, Yoshiro Terawaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Pseudomonas aeruginosa cytotoxin is a protein toxin exhibiting cytotoxic effects on various eukaryotic cells. The toxin was purified from a crude extract of Pseudomonas aeruginosa 158 by trypsin treatment and serial chromatography. The purified cytotoxin was electrophoresed as a single protein band on polyacrylamide gel electrophoresis (PAGE) in the presence or absence of sodium dodecyl sulfate (SDS). The molecular weight of the purified toxin was determined to be 29000 by SDS-PAGE and gel filtration chromatography in the presence of 6 m guanidine hydrochloride; the isoelectoric point was pH 6.0. The N-terminal amino acid sequence of the purified toxin was determined. The purified toxin showed the strongest cytotoxic effect on rabbit polymorphonuclear leukocytes and diverse degrees of cytotoxic effects on various eukaryotic cells including red blood cells and cultured cells.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalMicrobial Pathogenesis
Volume6
Issue number2
DOIs
Publication statusPublished - Feb 1989

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Cytotoxins
Eukaryotic Cells
Complex Mixtures
Sodium Dodecyl Sulfate
Pseudomonas aeruginosa
Polyacrylamide Gel Electrophoresis
Guanidine
Trypsin
Gel Chromatography
Chromatography
Amino Acid Sequence
Cultured Cells
Proteins
Neutrophils
Erythrocytes
Molecular Weight
Rabbits
Pseudomonas aeruginosa Cytotoxins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

Purification and characterization of cytotoxin from the crude extract of Pseudomonas aeruginosa. / Hayashi, Tetsuya; Kamio, Yoshiyuki; Terawaki, Yoshiro.

In: Microbial Pathogenesis, Vol. 6, No. 2, 02.1989, p. 103-112.

Research output: Contribution to journalArticle

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