Purification and characterization of nitrite-oxidizing enzyme from heterotrophic bacillus badius i-73, with special concern to catalase

Kenji Sakai, Hiroto Nisijima, Yoshihito Ikenaga, Mamoru Wakayama, Mitsuaki Moriguchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Nitrite-oxidizing enzyme I (NiOx I) was purified from a heterotrophic bacterium, Bacillus badius I-73. The enzyme was a homotetramer of a heme-containing peptide, and was similar to catalases from various sources in its N-terminal amino acid sequence. The purified enzyme also catalyzed H2O2 degradation. The nitrite oxidation reaction required ascorbic acid and oxygen. Successive H2O2 feeding could be substituted for ascorbic acid. These indicated that NiOx I is a catalase and nitrite was oxidized by a peroxidase-like reaction.

Original languageEnglish
Pages (from-to)2727-2730
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number12
DOIs
Publication statusPublished - Jun 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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