Purification and characterization of rab GDIβ from rat brain

Keishi Araki, Hiroyuki Nakanishi, Hisanobu Hirano, Masaki Kato, Takuya Sasaki, Yoshimi Takai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A new Rab GDI was purified from the synaptic soluble fraction of rat brain by several column chromatographies as a protein that inhibited the dissociation of [3H]GDP from Rab3A but was not recognized by an anti-Rab GDIα antibody. The partial amino acid sequence analysis revealed that it was identical with rat Rab GDIβ. Purified Rab GDIβ showed the kinetic properties similar to those of Rab GDIα, including the inhibitory effect on the dissociation of GDP from Rab3A, the substrate specificity, the requirement of the post-translational lipid modifications of Rab3A, the stoichiometric interaction with the GDP-bound form of Rab3A, the inhibitory effect on the binding of Rab3A to erythrocyte ghosts, and the stimulatory effect on the dissociation of Rab3A from the membrane.

Original languageEnglish
Pages (from-to)296-305
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume211
Issue number1
DOIs
Publication statusPublished - Jun 6 1995

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Purification
Rats
Brain
Column chromatography
Protein Sequence Analysis
Erythrocyte Membrane
Post Translational Protein Processing
Substrate Specificity
Chromatography
GDP dissociation inhibitor 1
Membranes
Lipids
Amino Acids
Kinetics
Antibodies
Substrates
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Purification and characterization of rab GDIβ from rat brain. / Araki, Keishi; Nakanishi, Hiroyuki; Hirano, Hisanobu; Kato, Masaki; Sasaki, Takuya; Takai, Yoshimi.

In: Biochemical and Biophysical Research Communications, Vol. 211, No. 1, 06.06.1995, p. 296-305.

Research output: Contribution to journalArticle

Araki, Keishi ; Nakanishi, Hiroyuki ; Hirano, Hisanobu ; Kato, Masaki ; Sasaki, Takuya ; Takai, Yoshimi. / Purification and characterization of rab GDIβ from rat brain. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 211, No. 1. pp. 296-305.
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