Purification and partial identification of bacteriocin ISK-1, a new lantibiotic produced by pediococcus sp. ISK-1

Hirokazu Kimura, Hiromi Matsusaki, Toshihiro Sashihara, Kenji Sonomoto, Ayaaki Ishizaki

Research output: Contribution to journalArticle

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Abstract

Bacteriocin ISK-1 is a proteinaceous inhibitory substance produced by Pediococcus sp. ISK-1 isolated from well-aged Nukadoko. Bacteriocin ISK-1 was purified by acid treatment, ammonium sulfate precipitation, cation-exchange chromatography, and reversed-phase HPLC from the culture supernatant of Pediococcus sp. ISK-1. Purification of bacteriocin ISK-1 resulted in a 30-fold increase in the specific activity and the recovery was 17%. Molecular mass of bacteriocin ISK-1 measured by fast atom bombardment-mass spectrometry was 2,960. The amino acid composition analysis of bacteriocin ISK-1 showed that it contained unusual amino acids such as lanthionine and/or 3-methyllanthionine, which is a characteristic of lantibiotics. The N-terminal amino acid sequence analysis indicated the first seven N-terminal amino acid residues as NH2-K-K-K-S-G-V-I. The primary sequence showed significant similarity to the lantibiotics lacticin 481 from Lactococcus lactis and variacin from Micrococcus varians, which suggests that bacteriocin ISK-1 is a novel lantibiotic belonging to a lacticin-481 type.

Original languageEnglish
Pages (from-to)2341-2345
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number12
DOIs
Publication statusPublished - Jan 1 1998

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Pediococcus
Bacteriocins
Purification
Amino Acids
Fast Atom Bombardment Mass Spectrometry
Micrococcus
Lactococcus lactis
Protein Sequence Analysis
Ammonium Sulfate
Molecular mass
Reverse-Phase Chromatography
Chromatography
Mass spectrometry
Cations
High Pressure Liquid Chromatography
Recovery

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Purification and partial identification of bacteriocin ISK-1, a new lantibiotic produced by pediococcus sp. ISK-1. / Kimura, Hirokazu; Matsusaki, Hiromi; Sashihara, Toshihiro; Sonomoto, Kenji; Ishizaki, Ayaaki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 12, 01.01.1998, p. 2341-2345.

Research output: Contribution to journalArticle

Kimura, Hirokazu ; Matsusaki, Hiromi ; Sashihara, Toshihiro ; Sonomoto, Kenji ; Ishizaki, Ayaaki. / Purification and partial identification of bacteriocin ISK-1, a new lantibiotic produced by pediococcus sp. ISK-1. In: Bioscience, Biotechnology and Biochemistry. 1998 ; Vol. 62, No. 12. pp. 2341-2345.
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