Abstract
Cellulomonas sp. isolated from soil produces a high level of α-mannosidase (α-mannanase) inductively in culture fluid. The enzyme had two different molecular weight forms, and the properties of the high-molecular-weight form were reported previously (Takegawa, K. et al.: Biochim. Biophys. Acta, 991, 431-437, 1989). The low-molecular-weight α-mannosidase was purified to homogeneity by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was over 150,000 by gel filtration. Unlike the high-molecular-weight form, the low-molecular-weight enzyme readily hydrolyzed α-1,2- and α-1,3-linked mannose chains.
Original language | English |
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Pages (from-to) | 129-131 |
Number of pages | 3 |
Journal | Journal of Fermentation and Bioengineering |
Volume | 69 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1990 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering