Purification and properties of a low-molecular-weight α-mannosidase from Cellulomonas sp.

Kaoru Takegawa, Satoshi Miki, Takayuki Jikibara, Shojiro Iwahara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cellulomonas sp. isolated from soil produces a high level of α-mannosidase (α-mannanase) inductively in culture fluid. The enzyme had two different molecular weight forms, and the properties of the high-molecular-weight form were reported previously (Takegawa, K. et al.: Biochim. Biophys. Acta, 991, 431-437, 1989). The low-molecular-weight α-mannosidase was purified to homogeneity by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was over 150,000 by gel filtration. Unlike the high-molecular-weight form, the low-molecular-weight enzyme readily hydrolyzed α-1,2- and α-1,3-linked mannose chains.

Original languageEnglish
Pages (from-to)129-131
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume69
Issue number2
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Cellulomonas
Mannosidases
Purification
Molecular Weight
Molecular weight
Enzymes
Gels
Mannose
Polyacrylates
Electrophoresis
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Soil
Soils
Fluids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Purification and properties of a low-molecular-weight α-mannosidase from Cellulomonas sp. / Takegawa, Kaoru; Miki, Satoshi; Jikibara, Takayuki; Iwahara, Shojiro.

In: Journal of Fermentation and Bioengineering, Vol. 69, No. 2, 1990, p. 129-131.

Research output: Contribution to journalArticle

Takegawa, Kaoru ; Miki, Satoshi ; Jikibara, Takayuki ; Iwahara, Shojiro. / Purification and properties of a low-molecular-weight α-mannosidase from Cellulomonas sp. In: Journal of Fermentation and Bioengineering. 1990 ; Vol. 69, No. 2. pp. 129-131.
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