Purification and properties of an enzyme oxidizing nitrite to nitrate from candida rugosa

Kenji Sakai, Koji Takano, Takashi Tachiki, Tatsurokuro Tochlkura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A nitrite-oxidizing enzyme was isolated (l300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220,000) consisted of four identical subunits with molecular weight of 58,000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.

Original languageEnglish
Pages (from-to)2783-2789
Number of pages7
JournalAgricultural and Biological Chemistry
Volume52
Issue number11
DOIs
Publication statusPublished - Jan 1 1988

Fingerprint

Candida rugosa
Candida
Nitrites
Nitrates
nitrites
Purification
nitrates
Enzymes
enzymes
Molecular Weight
Molecular weight
molecular weight
Dithionite
glucose oxidase
Glucose Oxidase
Catalase
Peroxidase
catalase
peroxidase
oxidation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Purification and properties of an enzyme oxidizing nitrite to nitrate from candida rugosa. / Sakai, Kenji; Takano, Koji; Tachiki, Takashi; Tochlkura, Tatsurokuro.

In: Agricultural and Biological Chemistry, Vol. 52, No. 11, 01.01.1988, p. 2783-2789.

Research output: Contribution to journalArticle

Sakai, Kenji ; Takano, Koji ; Tachiki, Takashi ; Tochlkura, Tatsurokuro. / Purification and properties of an enzyme oxidizing nitrite to nitrate from candida rugosa. In: Agricultural and Biological Chemistry. 1988 ; Vol. 52, No. 11. pp. 2783-2789.
@article{1d58416cda9347d19a1157618449b456,
title = "Purification and properties of an enzyme oxidizing nitrite to nitrate from candida rugosa",
abstract = "A nitrite-oxidizing enzyme was isolated (l300-fold purification, 15{\%} yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220,000) consisted of four identical subunits with molecular weight of 58,000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.",
author = "Kenji Sakai and Koji Takano and Takashi Tachiki and Tatsurokuro Tochlkura",
year = "1988",
month = "1",
day = "1",
doi = "10.1080/00021369.1988.10869156",
language = "English",
volume = "52",
pages = "2783--2789",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "11",

}

TY - JOUR

T1 - Purification and properties of an enzyme oxidizing nitrite to nitrate from candida rugosa

AU - Sakai, Kenji

AU - Takano, Koji

AU - Tachiki, Takashi

AU - Tochlkura, Tatsurokuro

PY - 1988/1/1

Y1 - 1988/1/1

N2 - A nitrite-oxidizing enzyme was isolated (l300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220,000) consisted of four identical subunits with molecular weight of 58,000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.

AB - A nitrite-oxidizing enzyme was isolated (l300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220,000) consisted of four identical subunits with molecular weight of 58,000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.

UR - http://www.scopus.com/inward/record.url?scp=0343373353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343373353&partnerID=8YFLogxK

U2 - 10.1080/00021369.1988.10869156

DO - 10.1080/00021369.1988.10869156

M3 - Article

AN - SCOPUS:0343373353

VL - 52

SP - 2783

EP - 2789

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 11

ER -