Purification and properties of chondroitinase produced by a bacterium isolated from soil

Kaoru Takegawa, Kenji Iwahara, Shojiro Iwahara

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6 Citations (Scopus)

Abstract

A Gram-positive bacterium isolated from a soil sample capable of growing on chondroitin sulfate as a carbon source has been isolated. From various bacteriological characteristics, the organism was identified as either an Aureobacterium or a Curtobacterium. When grown on chondroitin sulfate, the bacterium secreted a chondroitinase (EC 4.2.2.4 or EC 4.2.2.5) into the culture medium. The enzyme was purified to homogeneity as demonstrated by polyacrylamide gel electrophoresis and has an apparent molecular weight of about 81,000. It was inhibited by Hg2+, Zn2+, Fe2+ and Cu2+ ions. The enzyme hydrolyzed chondroitin sulfate-A, -C, chondroitin and hyaluronic acid, although the hydrolysis rate for hyaluronic acid was low. On the other hand, the enzyme did not act on iduronic acid-containing mucopolysaccharides such as dermatan sulfate, heparin and heparan sulfate. A decrease in viscosity and gel filtration analyses showed that the purified enzyme degrades the substrate endolytically at the initial reaction.

Original languageEnglish
Pages (from-to)128-131
Number of pages4
JournalJournal of Fermentation and Bioengineering
Volume72
Issue number2
DOIs
Publication statusPublished - Jan 1 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

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