Purification and Some Properties of an Antibacterial Factor Derived from Bacillus subtilis FHC 402

Takahisa Miyamoto, Makoto Yoshimoto, Koji Yamada, Shoji Hatano, Masakazu Tsutsumi

Research output: Contribution to journalArticle

Abstract

Bacillus subtilis FHC 402-derived antibacterial factor (abbreviated to BAF), which showed antibacterial activity by its combined use with hexametaphosphate, was purified from a culture broth by means of column chromatography on octyl-Sepharose CL-4B, DEAE-Cellulofine AL and Bio-Gel P-6. BAF was purified 63-fold with an activity yield of 17%. The purified preparation gave a single band on the electrophoregram. BAF may be a glycopeptide containing 22 molecules of 8 different amino acids, 3 hexoses, 1 hexosamine, 1 deoxyribose, and unknown substances. The N-terminal amino acid of the BAF preparation was determined to be tyrosine and the C-terminal amino acid to be proline. Gel filtration estimated the molecular weight of the BAF preparation at 3,200. The purified BAF preparation was stable in a pH range from 5.0 to 11.0, was also stable on heating, but was unstable at pH 3.0.

Original languageEnglish
Pages (from-to)1169-1176
Number of pages8
JournalAgricultural and Biological Chemistry
Volume50
Issue number5
DOIs
Publication statusPublished - Jan 1 1986

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antibacterial properties
Bacilli
Bacillus subtilis
Purification
Amino Acids
amino acids
Gels
gels
hexosamines
Deoxyribose
glycopeptides
Hexosamines
Column chromatography
Hexoses
Glycopeptides
hexoses
Proline
Heating
agarose
Gel Chromatography

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Purification and Some Properties of an Antibacterial Factor Derived from Bacillus subtilis FHC 402. / Miyamoto, Takahisa; Yoshimoto, Makoto; Yamada, Koji; Hatano, Shoji; Tsutsumi, Masakazu.

In: Agricultural and Biological Chemistry, Vol. 50, No. 5, 01.01.1986, p. 1169-1176.

Research output: Contribution to journalArticle

Miyamoto, Takahisa ; Yoshimoto, Makoto ; Yamada, Koji ; Hatano, Shoji ; Tsutsumi, Masakazu. / Purification and Some Properties of an Antibacterial Factor Derived from Bacillus subtilis FHC 402. In: Agricultural and Biological Chemistry. 1986 ; Vol. 50, No. 5. pp. 1169-1176.
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