Purification of extracellular lacease from rhizoctonia praticola

Jerzy Rogalski, Grzegorz Janusz, Dorota Legiec, C. H O Nam-Seok, Soo Jeong Shin, Shoji Ohga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rhizoctonia praticola was found to produce large amounts of extracellular lacease when grown aerobically on the optimized Lindenberg and Holm medium in fermenter culture with automatic pH control. The lacease from this source was purified to homogeneity by a rapid procedure, using ion-exchange chromatography, affinity chromatography and chromatofocussing. The enzymes isoforms were recovered with a 38- to 115-fold increase in specific activity and a yield for lacl=38.93%; lac2 = 14.71% and lac3 = 7.65%. The molecular weight of the purified enzymes proved to be 215, 175 and 68 kDa respectively as determined by size-exclusion HPLC. The isoelectric points were between 6.6 and 7.8, and the carbohydrate content in the purified enzymes was between 6.4-9.7%.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume56
Issue number1
Publication statusPublished - Feb 2011

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Rhizoctonia
Thanatephorus cucumeris
Enzymes
enzymes
fermenters
Ion Exchange Chromatography
Isoelectric Point
ion exchange chromatography
affinity chromatography
isoelectric point
carbohydrate content
Affinity Chromatography
Protein Isoforms
high performance liquid chromatography
Molecular Weight
High Pressure Liquid Chromatography
Carbohydrates
molecular weight

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Biotechnology

Cite this

Rogalski, J., Janusz, G., Legiec, D., Nam-Seok, C. H. O., Shin, S. J., & Ohga, S. (2011). Purification of extracellular lacease from rhizoctonia praticola. Journal of the Faculty of Agriculture, Kyushu University, 56(1), 1-7.

Purification of extracellular lacease from rhizoctonia praticola. / Rogalski, Jerzy; Janusz, Grzegorz; Legiec, Dorota; Nam-Seok, C. H O; Shin, Soo Jeong; Ohga, Shoji.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 56, No. 1, 02.2011, p. 1-7.

Research output: Contribution to journalArticle

Rogalski, J, Janusz, G, Legiec, D, Nam-Seok, CHO, Shin, SJ & Ohga, S 2011, 'Purification of extracellular lacease from rhizoctonia praticola', Journal of the Faculty of Agriculture, Kyushu University, vol. 56, no. 1, pp. 1-7.
Rogalski J, Janusz G, Legiec D, Nam-Seok CHO, Shin SJ, Ohga S. Purification of extracellular lacease from rhizoctonia praticola. Journal of the Faculty of Agriculture, Kyushu University. 2011 Feb;56(1):1-7.
Rogalski, Jerzy ; Janusz, Grzegorz ; Legiec, Dorota ; Nam-Seok, C. H O ; Shin, Soo Jeong ; Ohga, Shoji. / Purification of extracellular lacease from rhizoctonia praticola. In: Journal of the Faculty of Agriculture, Kyushu University. 2011 ; Vol. 56, No. 1. pp. 1-7.
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