Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus

Chisaka Osamu, Araki Kimi, Ochiya Takahiro, Tsurimoto Toshiki, Hiranyawasitte Attatippaholkun Wacharee, Yanaihara Noboru, Matsubara Kenichi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalGene
Volume60
Issue number2-3
DOIs
Publication statusPublished - 1987

All Science Journal Classification (ASJC) codes

  • Genetics

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