Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus

Chisaka Osamu, Araki Kimi, Ochiya Takahiro, Toshiki Tsurimoto, Hiranyawasitte Attatippaholkun Wacharee, Yanaihara Noboru, Matsubara Kenichi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalGene
Volume60
Issue number2-3
DOIs
Publication statusPublished - Jan 1 1987

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Hepatoblastoma
Hepatitis B virus
Escherichia coli
Cell Line
Virion
Genes
Nuclear Envelope
Ion Exchange Chromatography
Open Reading Frames
Antibodies

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus. / Osamu, Chisaka; Kimi, Araki; Takahiro, Ochiya; Tsurimoto, Toshiki; Wacharee, Hiranyawasitte Attatippaholkun; Noboru, Yanaihara; Kenichi, Matsubara.

In: Gene, Vol. 60, No. 2-3, 01.01.1987, p. 183-189.

Research output: Contribution to journalArticle

Osamu, Chisaka ; Kimi, Araki ; Takahiro, Ochiya ; Tsurimoto, Toshiki ; Wacharee, Hiranyawasitte Attatippaholkun ; Noboru, Yanaihara ; Kenichi, Matsubara. / Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus. In: Gene. 1987 ; Vol. 60, No. 2-3. pp. 183-189.
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