Purification of phosphatidylglycerophosphate synthase from Chinese hamster ovary cells

K. Kawasaki, Osamu Kuge, Y. Yamakawa, M. Nishijima

Research output: Contribution to journalArticle

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Abstract

Phosphatidylglycerophosphate (PGP) synthase catalyses the committed step in the biosynthesis of phosphatidylglycerol and cardiolipin in mammalian cells. Recently we isolated a Chinese hamster ovary (CHO) PGS1 cDNA encoding PGP synthase. In the present study we purified this PGP synthase to near-homogeneity from the mitochondrial fraction of CHO-K1 cells; the final enzyme preparation gave a single 60 kDa protein on SDS/PAGE. Polyclonal antibodies raised against a recombinant CHO PGS1 protein cross-reacted with the purified 60 kDa protein and with CHO membrane proteins of 60 kDa and 62 kDa that increased after transfection with the PGS1 cDNA. The 60 and 62 kDa protein levels in a PGP synthase-defective mutant of CHO-K1 cells were markedly lower than those in CHO-K1 cells. These results indicated that the purified 60 kDa protein was PGP synthase encoded by the PGS1 gene. In addition we found that the purified PGP synthase had no PGP phosphatase activity, indicating that phosphatidylglycerol was produced from CDP-diacylglycerol through two steps catalysed by distinct enzymes, PGP synthase and PGP phosphatase.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalBiochemical Journal
Volume354
Issue number1
DOIs
Publication statusPublished - Feb 15 2001

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CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase
Cricetulus
Purification
Ovary
Cells
Phosphatidylglycerols
Proteins
Phosphoric Monoester Hydrolases
Cytidine Diphosphate Diglycerides
Complementary DNA
Cardiolipins
Biosynthesis
Enzymes
Transfection
Polyacrylamide Gel Electrophoresis
Membrane Proteins
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Purification of phosphatidylglycerophosphate synthase from Chinese hamster ovary cells. / Kawasaki, K.; Kuge, Osamu; Yamakawa, Y.; Nishijima, M.

In: Biochemical Journal, Vol. 354, No. 1, 15.02.2001, p. 9-15.

Research output: Contribution to journalArticle

Kawasaki, K. ; Kuge, Osamu ; Yamakawa, Y. ; Nishijima, M. / Purification of phosphatidylglycerophosphate synthase from Chinese hamster ovary cells. In: Biochemical Journal. 2001 ; Vol. 354, No. 1. pp. 9-15.
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