TY - JOUR
T1 - Purified bacteriophage λ O protein binds to four repeating sequences at the λ replication origin
AU - Tsurimoto, Toshiki
AU - Matsubara, Kenichi
N1 - Funding Information:
We would like to express our gratitudes to Bruce Alberts for his critical reading of the manuscript. This work was supported by Scientific Research grants from the Ministry of Education, Science and Culture of Japan.
PY - 1981/4/24
Y1 - 1981/4/24
N2 - The bacteriophage λ 0 protein is needed for initiation of λ DNA replication. Several lines of evidence suggest that initiation requires that this protein interacts with a specific sequence called ori (for origin) in λ DNA. We have purified this protein to near homogeneity and studied the protection against nuclease cleavage of the origin DNA sequences. Our data demonstrate that the 0 protein binds within an interval of about 95 base pairs (bp), which contains four tandemly arranged 19bp repeating sequences, ATCCCTCAAAACGA(G)GG GAT(A). At a low concentration of 0 protein, the inner two repeats are primarily covered, while binding to the outer two repeats requires a high concentration of 0 protein. From the molecular size of 0 protein (32,000 daltons), and the internal symmetry in each 19bp repeat, we inferred that the 0 protein may bind in dimeric form, and that the 95bp region may be filled only when four such dimers have bound. This interaction is discussed in connection with the "activation" of the ori by 0 protein leading to initiation of DNA synthesis.
AB - The bacteriophage λ 0 protein is needed for initiation of λ DNA replication. Several lines of evidence suggest that initiation requires that this protein interacts with a specific sequence called ori (for origin) in λ DNA. We have purified this protein to near homogeneity and studied the protection against nuclease cleavage of the origin DNA sequences. Our data demonstrate that the 0 protein binds within an interval of about 95 base pairs (bp), which contains four tandemly arranged 19bp repeating sequences, ATCCCTCAAAACGA(G)GG GAT(A). At a low concentration of 0 protein, the inner two repeats are primarily covered, while binding to the outer two repeats requires a high concentration of 0 protein. From the molecular size of 0 protein (32,000 daltons), and the internal symmetry in each 19bp repeat, we inferred that the 0 protein may bind in dimeric form, and that the 95bp region may be filled only when four such dimers have bound. This interaction is discussed in connection with the "activation" of the ori by 0 protein leading to initiation of DNA synthesis.
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U2 - 10.1093/nar/9.8.1789
DO - 10.1093/nar/9.8.1789
M3 - Article
C2 - 6264392
AN - SCOPUS:0019448275
VL - 9
SP - 1789
EP - 1799
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 8
ER -