Putative "stemness" gene Jam-B is not required for maintenance of stem cell state in embryonic, neural, or hematopoietic stem cells

Takehisa Sakaguchi, Masazumi Nishimoto, Satoru Miyagi, Atsushi Iwama, Yohei Morita, Naoki Iwamori, Hiromitsu Nakauchi, Hiroshi Kiyonari, Masami Muramatsu, Akihiko Okuda

Research output: Contribution to journalArticle

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Abstract

Many genes have been identified that are specifically expressed in multiple types of stem cells in their undifferentiated state. It is generally assumed that at least some of these putative "stemness" genes are involved in maintaining properties that are common to all stem cells. We compared gene expression profiles between undifferentiated and differentiated embryonic stem cells (ESCs) using DNA microarrays. We identified several genes with much greater signal in undifferentiated ESCs than in their differentiated derivatives, among them the putative sternness gene encoding junctional adhesion molecule B (Jam-B gene). However, in spite of the specific expression in undifferentiated ESCs, Jam-B mutant ESCs had normal morphology and pluripotency. Furthermore, Jam-B homozygous mutant mice are fertile and have no overt developmental defects. Moreover, we found that neural and hematopoietic stem cells recovered from Jam-B mutant mice are not impaired in their ability to self-renew and differentiate. These results demonstrate that Jam-B is dispensable for normal mouse development and stem cell identity in embryonic, neural, and hematopoietic stem cells.

Original languageEnglish
Pages (from-to)6557-6570
Number of pages14
JournalMolecular and cellular biology
Volume26
Issue number17
DOIs
Publication statusPublished - Sep 1 2006

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Neural Stem Cells
Embryonic Stem Cells
Hematopoietic Stem Cells
Stem Cells
Maintenance
Genes
Junctional Adhesion Molecule B
Oligonucleotide Array Sequence Analysis
Transcriptome

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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Putative "stemness" gene Jam-B is not required for maintenance of stem cell state in embryonic, neural, or hematopoietic stem cells. / Sakaguchi, Takehisa; Nishimoto, Masazumi; Miyagi, Satoru; Iwama, Atsushi; Morita, Yohei; Iwamori, Naoki; Nakauchi, Hiromitsu; Kiyonari, Hiroshi; Muramatsu, Masami; Okuda, Akihiko.

In: Molecular and cellular biology, Vol. 26, No. 17, 01.09.2006, p. 6557-6570.

Research output: Contribution to journalArticle

Sakaguchi, T, Nishimoto, M, Miyagi, S, Iwama, A, Morita, Y, Iwamori, N, Nakauchi, H, Kiyonari, H, Muramatsu, M & Okuda, A 2006, 'Putative "stemness" gene Jam-B is not required for maintenance of stem cell state in embryonic, neural, or hematopoietic stem cells', Molecular and cellular biology, vol. 26, no. 17, pp. 6557-6570. https://doi.org/10.1128/MCB.00729-06
Sakaguchi, Takehisa ; Nishimoto, Masazumi ; Miyagi, Satoru ; Iwama, Atsushi ; Morita, Yohei ; Iwamori, Naoki ; Nakauchi, Hiromitsu ; Kiyonari, Hiroshi ; Muramatsu, Masami ; Okuda, Akihiko. / Putative "stemness" gene Jam-B is not required for maintenance of stem cell state in embryonic, neural, or hematopoietic stem cells. In: Molecular and cellular biology. 2006 ; Vol. 26, No. 17. pp. 6557-6570.
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