Pyrene excimer-based dual-emission detection of a oligoaspartate tag-fused protein by using a ZnII-DpaTyr probe

Kei Honda, Sho Hei Fujishima, Akio Ojida, Itaru Hamachi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A dual-emission sensing system for proteins tethered to a repeated tetra-aspartate tag (D4-tag) was successfully developed by using the binding-induced excimer formation of the pyrene-appended ZnII-DpaTyr probe. This allows selective detection of the D4-tag-fused protein in a simple and convenient manner by the naked eye. (Figure Presented)

Original languageEnglish
Pages (from-to)1370-1372
Number of pages3
JournalChemBioChem
Volume8
Issue number12
DOIs
Publication statusPublished - Aug 13 2007
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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