QCM-D analysis of material-cell interactions targeting a single cell during initial cell attachment

Etsuko Watarai, Ryosuke Matsuno, Tomohiro Konno, Kazuhiko Ishihara, Madoka Takai

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Clarifying material-cell interactions after eliminating various effects including cell-cell interactions is a very important issue in tissue and cell engineering. We investigated several cellular behaviors dynamically after growing cells individually one by one to eliminate cell-cell contact by direct observation using the quartz crystal microbalance with dissipation (QCM-D) and microscopy. The initial cellular behaviors of several cells (adsorption, attachment and spreading of L929 mouse fibroblasts) on the gold electrode of the QCM-D were classified into three regions according to the slope of the Df plots: I, cell adsorption and desorption; II, attachment and spreading; and III, secretion of microexudates. When the number of cells increased which leads to increase of cell-cell distance, region III disappeared and the slope of the Df plots in region II became steeper. The behavior related to the variation in the number of attached cells was indicated by the adhesion strength between the cells and the gold electrode. Furthermore, the slope of the Df plots was not shown on the (poly(2-methacryloyloxyethyl phosphorylcholine)-co-2- (methacryloyloxy)ethylthiol)-modified gold electrode and almost all of the cells were not attached to the polymer surface. The initial cell attachment behavior, especially the strength of cell adhesion to the material surface, was evaluated quantitatively by determining the slope of the Df plots using the QCM-D system.

Original languageEnglish
Pages (from-to)1297-1302
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume171-172
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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