Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation

Tomoko Kobayashi; Yoshiki Koizumi; Junko S. Takeuchi; Naoko Misawa; Yuichi Kimura; Satoru Morita; Kazuyuki Aihara; Yoshio Koyanagi; Shingo Iwami; Kei Sato

doi:10.1128/JVI.00062-14

Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation

Tomoko Kobayashi, Yoshiki Koizumi, Junko S. Takeuchi, Naoko Misawa, Yuichi Kimura, Satoru Morita, Kazuyuki Aihara, Yoshio Koyanagi, Shingo Iwami, Kei Sato

dynamic biology

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

APOBEC3F and APOBEC3G cytidine deaminases potently inhibit human immunodeficiency virus type 1 (HIV-1) replication by enzymatically inserting G-to-A mutations in viral DNA and/or impairing viral reverse transcription independently of their deaminase activity. Through experimental and mathematical investigation, here we quantitatively demonstrate that 99.3% of the antiviral effect of APOBEC3G is dependent on its deaminase activity, whereas 30.2% of the antiviral effect of APOBEC3F is attributed to deaminase-independent ability. This is the first report quantitatively elucidating how APOBEC3F and APOBEC3G differ in their anti-HIV-1 modes.

Original language	English
Pages (from-to)	5881-5887
Number of pages	7
Journal	Journal of virology
Volume	88
Issue number	10
DOIs	https://doi.org/10.1128/JVI.00062-14
Publication status	Published - 2014

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Insect Science
Virology

UN SDGs

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10.1128/JVI.00062-14

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Kobayashi, T., Koizumi, Y., Takeuchi, J. S., Misawa, N., Kimura, Y., Morita, S., Aihara, K., Koyanagi, Y., Iwami, S., & Sato, K. (2014). Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation. Journal of virology, 88(10), 5881-5887. https://doi.org/10.1128/JVI.00062-14

Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation. / Kobayashi, Tomoko; Koizumi, Yoshiki; Takeuchi, Junko S. et al.

In: Journal of virology, Vol. 88, No. 10, 2014, p. 5881-5887.

Research output: Contribution to journal › Article › peer-review

Kobayashi, T, Koizumi, Y, Takeuchi, JS, Misawa, N, Kimura, Y, Morita, S, Aihara, K, Koyanagi, Y, Iwami, S & Sato, K 2014, 'Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation', Journal of virology, vol. 88, no. 10, pp. 5881-5887. https://doi.org/10.1128/JVI.00062-14

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abstract = "APOBEC3F and APOBEC3G cytidine deaminases potently inhibit human immunodeficiency virus type 1 (HIV-1) replication by enzymatically inserting G-to-A mutations in viral DNA and/or impairing viral reverse transcription independently of their deaminase activity. Through experimental and mathematical investigation, here we quantitatively demonstrate that 99.3% of the antiviral effect of APOBEC3G is dependent on its deaminase activity, whereas 30.2% of the antiviral effect of APOBEC3F is attributed to deaminase-independent ability. This is the first report quantitatively elucidating how APOBEC3F and APOBEC3G differ in their anti-HIV-1 modes.",

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AU - Kimura, Yuichi

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AU - Aihara, Kazuyuki

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Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation

Abstract

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