Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation

Tomoko Kobayashi, Yoshiki Koizumi, Junko S. Takeuchi, Naoko Misawa, Yuichi Kimura, Satoru Morita, Kazuyuki Aihara, Yoshio Koyanagi, Shingo Iwami, Kei Sato

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26 Citations (Scopus)

Abstract

APOBEC3F and APOBEC3G cytidine deaminases potently inhibit human immunodeficiency virus type 1 (HIV-1) replication by enzymatically inserting G-to-A mutations in viral DNA and/or impairing viral reverse transcription independently of their deaminase activity. Through experimental and mathematical investigation, here we quantitatively demonstrate that 99.3% of the antiviral effect of APOBEC3G is dependent on its deaminase activity, whereas 30.2% of the antiviral effect of APOBEC3F is attributed to deaminase-independent ability. This is the first report quantitatively elucidating how APOBEC3F and APOBEC3G differ in their anti-HIV-1 modes.

Original languageEnglish
Pages (from-to)5881-5887
Number of pages7
JournalJournal of virology
Volume88
Issue number10
DOIs
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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