Quantification of Periodontal Pathogens Cell Counts by Capillary Electrophoresis

Zhenqing Li, Shaoxiong Chen, Chenchen Liu, Dawei Zhang, Xiaoming Dou, Yoshinori Yamaguchi

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Gingivitis is a highly prevalent periodontal disease around the worldwide. Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f) were considered to be three important periodontal pathogens related to gingivitis, and research shows that the counts of periodontal pathogen cells in the patients before, during, and after fixed orthodontic appliance therapy were quite different. We proposed a simple method to extract the periodontal pathogens from the periodontal pocket in this work and demonstrated a new approach to determine periodontal pathogen level based on capillary electrophoresis (CE). After polymerase chain reaction amplification of P.g (197bp), T.d (311bp), and T.f (641bp), it shows that they can rapidly identified by CE within 5min. The peak area in the eletropherogram is linearly related to the concentration of P.g, T.d, and T.f, and the correlation coefficients R2 corresponding to them are 0.993, 0.993, and 0.956, respectively. According to this linearly relationship, the estimated concentration of P.g, T.d, and T.f in gingival crevicular fluid from one volunteer was inferred to be about 9.90×102, 1.48×103, and 9.01×102cells/μl, respectively.

Original languageEnglish
Pages (from-to)286-290
Number of pages5
JournalJournal of Chromatography A
Volume1361
DOIs
Publication statusPublished - Sep 26 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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