Quantification of trans-1,4-polyisoprene in Eucommia ulmoides by fourier transform infrared spectroscopy and pyrolysis-gas chromatography/mass spectrometry

Shinya Takeno; Takeshi Bamba; Yoshihisa Nakazawa; Eiichiro Fukusaki; Atsushi Okazawa; Akio Kobayashi

doi:10.1263/jbb.105.355

Quantification of trans-1,4-polyisoprene in Eucommia ulmoides by fourier transform infrared spectroscopy and pyrolysis-gas chromatography/mass spectrometry

Shinya Takeno, Takeshi Bamba, Yoshihisa Nakazawa, Eiichiro Fukusaki, Atsushi Okazawa, Akio Kobayashi

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Commercial development of trans-1,4-polyisoprene from Eucommia ulmoides Oliver (EU-rubber) requires specific knowledge on selection of high-rubber-content lines and establishment of agronomic cultivation methods for achieving maximum EU-rubber yield. The development can be facilitated by high-throughput and highly sensitive analytical techniques for EU-rubber extraction and quantification. In this paper, we described an efficient EU-rubber extraction method, and validated that the accuracy was equivalent to that of the conventional Soxhlet extraction method. We also described a highly sensitive quantification method for EU-rubber by Fourier transform infrared spectroscopy (FT-IR) and pyrolysis-gas chromatography/mass spectrometry (PyGC/MS). We successfully applied the extraction/quantification method for study of seasonal changes in EU-rubber content and molecular weight distribution.

Original language	English
Pages (from-to)	355-359
Number of pages	5
Journal	Journal of Bioscience and Bioengineering
Volume	105
Issue number	4
DOIs	https://doi.org/10.1263/jbb.105.355
Publication status	Published - Apr 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Quantification of trans-1,4-polyisoprene in Eucommia ulmoides by fourier transform infrared spectroscopy and pyrolysis-gas chromatography/mass spectrometry",

abstract = "Commercial development of trans-1,4-polyisoprene from Eucommia ulmoides Oliver (EU-rubber) requires specific knowledge on selection of high-rubber-content lines and establishment of agronomic cultivation methods for achieving maximum EU-rubber yield. The development can be facilitated by high-throughput and highly sensitive analytical techniques for EU-rubber extraction and quantification. In this paper, we described an efficient EU-rubber extraction method, and validated that the accuracy was equivalent to that of the conventional Soxhlet extraction method. We also described a highly sensitive quantification method for EU-rubber by Fourier transform infrared spectroscopy (FT-IR) and pyrolysis-gas chromatography/mass spectrometry (PyGC/MS). We successfully applied the extraction/quantification method for study of seasonal changes in EU-rubber content and molecular weight distribution.",

author = "Shinya Takeno and Takeshi Bamba and Yoshihisa Nakazawa and Eiichiro Fukusaki and Atsushi Okazawa and Akio Kobayashi",

note = "Funding Information: This work was partly supported by the New Energy and Industrial Technology Development Organization (NEDO). The authors are very grateful to Dr. Y. Su (College of Forestry, Northwest Agricultural and Forestry University, Yangling, Shannxi, China) for providing the leaves from various E. ulmoides trees, and Dr. L. Tarachiwin and Miss K. Jumtee (Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan) for helpful discussions.",

year = "2008",

month = apr,

doi = "10.1263/jbb.105.355",

language = "English",

volume = "105",

pages = "355--359",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "The Society for Biotechnology, Japan",

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AU - Takeno, Shinya

AU - Bamba, Takeshi

AU - Nakazawa, Yoshihisa

AU - Fukusaki, Eiichiro

AU - Okazawa, Atsushi

AU - Kobayashi, Akio

N1 - Funding Information: This work was partly supported by the New Energy and Industrial Technology Development Organization (NEDO). The authors are very grateful to Dr. Y. Su (College of Forestry, Northwest Agricultural and Forestry University, Yangling, Shannxi, China) for providing the leaves from various E. ulmoides trees, and Dr. L. Tarachiwin and Miss K. Jumtee (Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan) for helpful discussions.

PY - 2008/4

Y1 - 2008/4

N2 - Commercial development of trans-1,4-polyisoprene from Eucommia ulmoides Oliver (EU-rubber) requires specific knowledge on selection of high-rubber-content lines and establishment of agronomic cultivation methods for achieving maximum EU-rubber yield. The development can be facilitated by high-throughput and highly sensitive analytical techniques for EU-rubber extraction and quantification. In this paper, we described an efficient EU-rubber extraction method, and validated that the accuracy was equivalent to that of the conventional Soxhlet extraction method. We also described a highly sensitive quantification method for EU-rubber by Fourier transform infrared spectroscopy (FT-IR) and pyrolysis-gas chromatography/mass spectrometry (PyGC/MS). We successfully applied the extraction/quantification method for study of seasonal changes in EU-rubber content and molecular weight distribution.

AB - Commercial development of trans-1,4-polyisoprene from Eucommia ulmoides Oliver (EU-rubber) requires specific knowledge on selection of high-rubber-content lines and establishment of agronomic cultivation methods for achieving maximum EU-rubber yield. The development can be facilitated by high-throughput and highly sensitive analytical techniques for EU-rubber extraction and quantification. In this paper, we described an efficient EU-rubber extraction method, and validated that the accuracy was equivalent to that of the conventional Soxhlet extraction method. We also described a highly sensitive quantification method for EU-rubber by Fourier transform infrared spectroscopy (FT-IR) and pyrolysis-gas chromatography/mass spectrometry (PyGC/MS). We successfully applied the extraction/quantification method for study of seasonal changes in EU-rubber content and molecular weight distribution.

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Quantification of trans-1,4-polyisoprene in Eucommia ulmoides by fourier transform infrared spectroscopy and pyrolysis-gas chromatography/mass spectrometry

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