Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture

Hiroki Ikeda, Ana Godinho-Santos, Sylvie Rato, Bénédicte Vanwalscappel, François Clavel, Kazuyuki Aihara, Shingo Iwami, Fabrizio Mammano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4- and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses.

Original languageEnglish
Article number11761
JournalScientific reports
Volume5
DOIs
Publication statusPublished - Jun 29 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture'. Together they form a unique fingerprint.

  • Cite this

    Ikeda, H., Godinho-Santos, A., Rato, S., Vanwalscappel, B., Clavel, F., Aihara, K., Iwami, S., & Mammano, F. (2015). Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture. Scientific reports, 5, [11761]. https://doi.org/10.1038/srep11761