Quantitation of HLA-A *0201 bound tumor associated antigens on a peptide pulsed B cell line

Hiroshi Wataya, Nobuhiro Kamikawaji, Yoichi Nakanishi, Koichi Takayama, Nobuyuki Hara, Takehiko Sasazuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

CTLs recognize 8- to 10-mer peptides on MHC class I molecules. Recent studies have shown that human CTLs kill autologous tumor cells in an HLA-restricted and peptide-specific manner, and that artificial peptides can stimulate tumor-specific CTLs both in vitro and in vivo. Accordingly, several human clinical trials using such peptides are ongoing worldwide. In such methods, the amount of peptide-MHC complexes that remain on the cell surface of APCs after peptide administration is crucial, because CTL activation depends on the number of ligated TCRs and co-stimulation. However, it remains uncertain how many peptide-MHC complexes are reconstituted and remain on live cells after peptide administration. We herein examined the binding affinities of five HLA-A*0201 restricted peptides - four TAAs and one HIV antigen - to HLA-A*0201 molecules and their decay rates on a live B cell line using tandem mass spectrometry. Our experiments showed that nearly 105 peptide-MHC complexes per cell could be reconstituted on a cell surface by pulsing a high dose of peptide even if the binding affinities were intermediate or low. However, the decay rates observed for these peptide-MHC complexes on a B cell line were faster than previously estimated.

Original languageEnglish
Pages (from-to)125-132
Number of pages8
JournalHuman Immunology
Volume62
Issue number2
DOIs
Publication statusPublished - Mar 6 2001

Fingerprint

Neoplasm Antigens
B-Lymphocytes
Cell Line
Peptides
peptide B
HLA-A*02:01 antigen
HIV Antigens
Tandem Mass Spectrometry
Neoplasms
Clinical Trials

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Wataya, H., Kamikawaji, N., Nakanishi, Y., Takayama, K., Hara, N., & Sasazuki, T. (2001). Quantitation of HLA-A *0201 bound tumor associated antigens on a peptide pulsed B cell line. Human Immunology, 62(2), 125-132. https://doi.org/10.1016/S0198-8859(00)00251-2

Quantitation of HLA-A *0201 bound tumor associated antigens on a peptide pulsed B cell line. / Wataya, Hiroshi; Kamikawaji, Nobuhiro; Nakanishi, Yoichi; Takayama, Koichi; Hara, Nobuyuki; Sasazuki, Takehiko.

In: Human Immunology, Vol. 62, No. 2, 06.03.2001, p. 125-132.

Research output: Contribution to journalArticle

Wataya, H, Kamikawaji, N, Nakanishi, Y, Takayama, K, Hara, N & Sasazuki, T 2001, 'Quantitation of HLA-A *0201 bound tumor associated antigens on a peptide pulsed B cell line', Human Immunology, vol. 62, no. 2, pp. 125-132. https://doi.org/10.1016/S0198-8859(00)00251-2
Wataya, Hiroshi ; Kamikawaji, Nobuhiro ; Nakanishi, Yoichi ; Takayama, Koichi ; Hara, Nobuyuki ; Sasazuki, Takehiko. / Quantitation of HLA-A *0201 bound tumor associated antigens on a peptide pulsed B cell line. In: Human Immunology. 2001 ; Vol. 62, No. 2. pp. 125-132.
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