Quantitation of tetrodotoxin in human plasma with mixed solvent and combined cartridges

Yukio Ohtsuka; Kou Takahashi; Yukiko Hino; Akiko Tsuji; Keiko Kudo; Noriaki Ikeda

Quantitation of tetrodotoxin in human plasma with mixed solvent and combined cartridges

Yukio Ohtsuka, Kou Takahashi, Yukiko Hino, Akiko Tsuji, Keiko Kudo, Noriaki Ikeda

Social Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

In order to improve the recovery of tetrodotoxin (TTX) in human plasma, our extraction and purification procedures were further evaluated. For the effective extraction of TTX, the use of mixed solvents, methanol-acetonitrile, with the ratio of about 8:2 was recommended. For the purification of TTX by the first cartridge, 3 kinds of cartridges, Sep-Pak C ₁₈, Sep-Pak tC ₁₈ and PS-2 were equally useful when complete removal of protein and lipids was carried out at the extraction step. In the purification of C ₉-base by the second cartridge, PS-2 gave best recovery with one-hour holding time. The established method should be useful for the trace analysis of TTX in human plasma.

Original language	English
Pages (from-to)	152-153
Number of pages	2
Journal	Japanese Journal of Forensic Toxicology
Volume	19
Issue number	2
Publication status	Published - Dec 1 2001

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Toxicology

Cite this

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abstract = "In order to improve the recovery of tetrodotoxin (TTX) in human plasma, our extraction and purification procedures were further evaluated. For the effective extraction of TTX, the use of mixed solvents, methanol-acetonitrile, with the ratio of about 8:2 was recommended. For the purification of TTX by the first cartridge, 3 kinds of cartridges, Sep-Pak C 18, Sep-Pak tC 18 and PS-2 were equally useful when complete removal of protein and lipids was carried out at the extraction step. In the purification of C 9-base by the second cartridge, PS-2 gave best recovery with one-hour holding time. The established method should be useful for the trace analysis of TTX in human plasma.",

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AU - Ohtsuka, Yukio

AU - Takahashi, Kou

AU - Hino, Yukiko

AU - Tsuji, Akiko

AU - Kudo, Keiko

AU - Ikeda, Noriaki

PY - 2001/12/1

Y1 - 2001/12/1

N2 - In order to improve the recovery of tetrodotoxin (TTX) in human plasma, our extraction and purification procedures were further evaluated. For the effective extraction of TTX, the use of mixed solvents, methanol-acetonitrile, with the ratio of about 8:2 was recommended. For the purification of TTX by the first cartridge, 3 kinds of cartridges, Sep-Pak C 18, Sep-Pak tC 18 and PS-2 were equally useful when complete removal of protein and lipids was carried out at the extraction step. In the purification of C 9-base by the second cartridge, PS-2 gave best recovery with one-hour holding time. The established method should be useful for the trace analysis of TTX in human plasma.

AB - In order to improve the recovery of tetrodotoxin (TTX) in human plasma, our extraction and purification procedures were further evaluated. For the effective extraction of TTX, the use of mixed solvents, methanol-acetonitrile, with the ratio of about 8:2 was recommended. For the purification of TTX by the first cartridge, 3 kinds of cartridges, Sep-Pak C 18, Sep-Pak tC 18 and PS-2 were equally useful when complete removal of protein and lipids was carried out at the extraction step. In the purification of C 9-base by the second cartridge, PS-2 gave best recovery with one-hour holding time. The established method should be useful for the trace analysis of TTX in human plasma.

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Quantitation of tetrodotoxin in human plasma with mixed solvent and combined cartridges

Abstract

All Science Journal Classification (ASJC) codes

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