Quantitative analysis and localization of mRNA transcripts of type I collagen, osteocalcin, MMP2, MMP 8, and MMP 13 during bone healing in a rat calvarial experimental defect model

Tomoko Itagaki, Takahiro Honma, Ichiro Takahashi, Seishi Echigo, Yasuyuki Sasano

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The study examined the expression of matrix metalloproteinases (MMPs), type I collagen and osteocalcin during bone healing in a rat calvarial experimental defect model. Twelve-week-old male Wistar rats were used. A full-thickness standardized trephine defect was made in the parietal bone, with the rat under anesthesia. RNA was extracted from tissue that filled the original bone defect on days 1 and 3 and in weeks 1, 2, 3, 5, 8, 10, 12, 18, and 24 and processed for quantitative analysis of expression of type I collagen, osteocalcin and matrix metalloproteinases (MMPs) 2, 8, and 13 by using real-time polymerase chain reaction. Alternatively, the rats were fixed by perfusion through the aorta and resected calvaria were processed for in situ hybridization for these molecules. The expression of type I collagen, osteocalcin and MMPs 2 and 13 increased toward week 2 and decreased thereafter, whereas the expression of MMP 8 was the highest on day 1. The mRNA transcripts of type I collagen and osteocalcin were localized in osteoblasts and osteocytes, some of which expressed MMPs 2, 8, and 13. Osteoblasts and osteocytes may play a role in the remodeling of extracellular matrices with MMPs during healing of a defect in bone.

Original languageEnglish
Pages (from-to)1038-1046
Number of pages9
JournalAnatomical Record
Volume291
Issue number8
DOIs
Publication statusPublished - Dec 1 2008
Externally publishedYes

Fingerprint

neutrophil collagenase
Matrix Metalloproteinase 8
Matrix Metalloproteinase 13
osteocalcin
collagen
Matrix Metalloproteinase 2
Osteocalcin
Collagen Type I
quantitative analysis
gelatinase A
defect
bone
Theoretical Models
bones
Bone and Bones
Osteocytes
Messenger RNA
matrix
osteoblasts
rats

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Biotechnology
  • Histology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Quantitative analysis and localization of mRNA transcripts of type I collagen, osteocalcin, MMP2, MMP 8, and MMP 13 during bone healing in a rat calvarial experimental defect model. / Itagaki, Tomoko; Honma, Takahiro; Takahashi, Ichiro; Echigo, Seishi; Sasano, Yasuyuki.

In: Anatomical Record, Vol. 291, No. 8, 01.12.2008, p. 1038-1046.

Research output: Contribution to journalArticle

@article{961a5a1329c546fdb0be9c2d5079b8dd,
title = "Quantitative analysis and localization of mRNA transcripts of type I collagen, osteocalcin, MMP2, MMP 8, and MMP 13 during bone healing in a rat calvarial experimental defect model",
abstract = "The study examined the expression of matrix metalloproteinases (MMPs), type I collagen and osteocalcin during bone healing in a rat calvarial experimental defect model. Twelve-week-old male Wistar rats were used. A full-thickness standardized trephine defect was made in the parietal bone, with the rat under anesthesia. RNA was extracted from tissue that filled the original bone defect on days 1 and 3 and in weeks 1, 2, 3, 5, 8, 10, 12, 18, and 24 and processed for quantitative analysis of expression of type I collagen, osteocalcin and matrix metalloproteinases (MMPs) 2, 8, and 13 by using real-time polymerase chain reaction. Alternatively, the rats were fixed by perfusion through the aorta and resected calvaria were processed for in situ hybridization for these molecules. The expression of type I collagen, osteocalcin and MMPs 2 and 13 increased toward week 2 and decreased thereafter, whereas the expression of MMP 8 was the highest on day 1. The mRNA transcripts of type I collagen and osteocalcin were localized in osteoblasts and osteocytes, some of which expressed MMPs 2, 8, and 13. Osteoblasts and osteocytes may play a role in the remodeling of extracellular matrices with MMPs during healing of a defect in bone.",
author = "Tomoko Itagaki and Takahiro Honma and Ichiro Takahashi and Seishi Echigo and Yasuyuki Sasano",
year = "2008",
month = "12",
day = "1",
doi = "10.1002/ar.20717",
language = "English",
volume = "291",
pages = "1038--1046",
journal = "Anatomical Record",
issn = "1932-8486",
publisher = "John Wiley and Sons Inc.",
number = "8",

}

TY - JOUR

T1 - Quantitative analysis and localization of mRNA transcripts of type I collagen, osteocalcin, MMP2, MMP 8, and MMP 13 during bone healing in a rat calvarial experimental defect model

AU - Itagaki, Tomoko

AU - Honma, Takahiro

AU - Takahashi, Ichiro

AU - Echigo, Seishi

AU - Sasano, Yasuyuki

PY - 2008/12/1

Y1 - 2008/12/1

N2 - The study examined the expression of matrix metalloproteinases (MMPs), type I collagen and osteocalcin during bone healing in a rat calvarial experimental defect model. Twelve-week-old male Wistar rats were used. A full-thickness standardized trephine defect was made in the parietal bone, with the rat under anesthesia. RNA was extracted from tissue that filled the original bone defect on days 1 and 3 and in weeks 1, 2, 3, 5, 8, 10, 12, 18, and 24 and processed for quantitative analysis of expression of type I collagen, osteocalcin and matrix metalloproteinases (MMPs) 2, 8, and 13 by using real-time polymerase chain reaction. Alternatively, the rats were fixed by perfusion through the aorta and resected calvaria were processed for in situ hybridization for these molecules. The expression of type I collagen, osteocalcin and MMPs 2 and 13 increased toward week 2 and decreased thereafter, whereas the expression of MMP 8 was the highest on day 1. The mRNA transcripts of type I collagen and osteocalcin were localized in osteoblasts and osteocytes, some of which expressed MMPs 2, 8, and 13. Osteoblasts and osteocytes may play a role in the remodeling of extracellular matrices with MMPs during healing of a defect in bone.

AB - The study examined the expression of matrix metalloproteinases (MMPs), type I collagen and osteocalcin during bone healing in a rat calvarial experimental defect model. Twelve-week-old male Wistar rats were used. A full-thickness standardized trephine defect was made in the parietal bone, with the rat under anesthesia. RNA was extracted from tissue that filled the original bone defect on days 1 and 3 and in weeks 1, 2, 3, 5, 8, 10, 12, 18, and 24 and processed for quantitative analysis of expression of type I collagen, osteocalcin and matrix metalloproteinases (MMPs) 2, 8, and 13 by using real-time polymerase chain reaction. Alternatively, the rats were fixed by perfusion through the aorta and resected calvaria were processed for in situ hybridization for these molecules. The expression of type I collagen, osteocalcin and MMPs 2 and 13 increased toward week 2 and decreased thereafter, whereas the expression of MMP 8 was the highest on day 1. The mRNA transcripts of type I collagen and osteocalcin were localized in osteoblasts and osteocytes, some of which expressed MMPs 2, 8, and 13. Osteoblasts and osteocytes may play a role in the remodeling of extracellular matrices with MMPs during healing of a defect in bone.

UR - http://www.scopus.com/inward/record.url?scp=52049105675&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52049105675&partnerID=8YFLogxK

U2 - 10.1002/ar.20717

DO - 10.1002/ar.20717

M3 - Article

C2 - 18615687

AN - SCOPUS:52049105675

VL - 291

SP - 1038

EP - 1046

JO - Anatomical Record

JF - Anatomical Record

SN - 1932-8486

IS - 8

ER -