Quantitative analysis of AML1/ET0 transcripts in peripheral blood stem cell harvests from patients with t(8;21) acute myelogenous leukaemia

Toshihiro Miyamoto, Koji Nagafuji, Mine Harada, Tetsuya Eto, Tomoaki Fujisaki, Akira Kubota, Koichi Akashi, Shin‐Ichi ‐I Mizuno, Katsuto Takenaka, Taisuke Kanaji, Hisashi Gondo, Takashi Okamura, Shoichi Inaba, Yoshiyuki Niho

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Abstract

Summary Peripheral blood stem cells (PBSC) have been used increasingly for haemopoietic reconstitution after marrow‐ablative chemotherapy in patients with acute leukaemia because of the possibility that there is a lower risk of leukaemic contamination. We have developed a titration assay using a competitive reverse transcriptase polymerase chain reaction (RT‐PCR) which is able to estimate the number of AML1/ETO transcripts so that minimal residual disease (MRD) can be monitored quantitatively in patients with t(8;21) acute myelogenous leukaemia (AML). Using a qualitative RT‐PCR method, AML1/ETO transcripts could be detected in all samples from 15 first PBSC harvests and 11 second PBSC harvests obtained from 15 patients with t(8;21) AML. With our competitive RT‐PCR assay, the number of AML1/ETO transcripts was found to be lower in the second PBSC harvest than that in the first in every individual. Furthermore, MRD in PBSC harvests was less than that in the corresponding bone marrow obtained on the day of PBSC collection in the individual patients studied. In 10 patients who received autologous blood stem cell transplantation (ABSCT), we could not find a relationship between the number of AML1/ETO transcripts in the infused PBSC harvests and the clinical outcome after ABSCT. The present study clearly indicates that although PBSC harvests collected after consolidation chemotherapy are contaminated by leukaemic cells, the degree of leukaemic contamination may decrease as chemotherapy is repeated. The mobilization of PBSC by repeated chemotherapy may provide an advantageous source of haemopoietic stem cells for ABSCT.

Original languageEnglish
Pages (from-to)132-138
Number of pages7
JournalBritish Journal of Haematology
Volume91
Issue number1
DOIs
Publication statusPublished - Sep 1995

All Science Journal Classification (ASJC) codes

  • Hematology

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