Quantitative analysis of cellular senescence phenotypes using an imaging cytometer

Miyako Udono, Keishi Kadooka, Shuntaro Yamashita, Yoshinori Katakura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Until now, various stimuli as well as serial passaging have been known to induce cellular senescence in normal human diploid fibroblasts. However, in many cases, we have encountered difficulty in quantitatively analyzing the cellular senescence phenotypes of senescent cells in a physiological condition. High-content screening (HCS)-based image analysis is becoming an important and powerful research tool. In the present study, an automated and quantitative cellular image-analysis system was employed to quantify the cellular senescence phenotypes induced in normal human diploid fibroblasts, TIG-1 cells, and found to be a powerful tool in the cellular senescence study.

Original languageEnglish
Pages (from-to)383-388
Number of pages6
JournalMethods
Volume56
Issue number3
DOIs
Publication statusPublished - Mar 1 2012

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Cell Aging
Fibroblasts
Image analysis
Phenotype
Imaging techniques
Diploidy
Chemical analysis
Screening
Research

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. / Udono, Miyako; Kadooka, Keishi; Yamashita, Shuntaro; Katakura, Yoshinori.

In: Methods, Vol. 56, No. 3, 01.03.2012, p. 383-388.

Research output: Contribution to journalArticle

Udono, Miyako ; Kadooka, Keishi ; Yamashita, Shuntaro ; Katakura, Yoshinori. / Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. In: Methods. 2012 ; Vol. 56, No. 3. pp. 383-388.
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