Quantitative analysis of digestion resistant ACE inhibitory dipeptides by small intestinal mucosa

Eiji Seki, Katsuhiro Osajima, Hiroshi Matsufuji, Toshiro Matsui, Yutaka Osajima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The short chain peptides derived from sardines were incubated for 120 min with an extract of porcine small intestinal mucosa. Digestion-resistant dipeptides found in the digest, Val-Tyr, Val-Trp, Ile-Tyr and Ile-Trp, inhibited angiotensin I converting enzyme (ACE). Amounts of the four dipeptides isolated from the digest using a Sep-pak C-18 cartridge column and three steps of high-performance liquid chromatography, were 85.88, 21.87, 10.69 and 6.51 mg/100 g peptides, respectively, and the contribution of four peptides to total ACE inhibitory activity was 10.5%. It was confirmed that ACE inhibitory dipeptides were present in the digest of the short chain peptides.

Original languageEnglish
Pages (from-to)967-969
Number of pages3
JournalNippon Shokuhin Kagaku Kogaku Kaishi
Volume43
Issue number8
DOIs
Publication statusPublished - Jan 1 1996

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dipeptides
Dipeptides
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
Intestinal Mucosa
intestinal mucosa
quantitative analysis
Digestion
digestion
peptides
Peptides
N-valyltryptophan
isoleucyl-tyrosine
valyltyrosine
sardines
Swine
high performance liquid chromatography
High Pressure Liquid Chromatography
swine
extracts

All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

Quantitative analysis of digestion resistant ACE inhibitory dipeptides by small intestinal mucosa. / Seki, Eiji; Osajima, Katsuhiro; Matsufuji, Hiroshi; Matsui, Toshiro; Osajima, Yutaka.

In: Nippon Shokuhin Kagaku Kogaku Kaishi, Vol. 43, No. 8, 01.01.1996, p. 967-969.

Research output: Contribution to journalArticle

Seki, Eiji ; Osajima, Katsuhiro ; Matsufuji, Hiroshi ; Matsui, Toshiro ; Osajima, Yutaka. / Quantitative analysis of digestion resistant ACE inhibitory dipeptides by small intestinal mucosa. In: Nippon Shokuhin Kagaku Kogaku Kaishi. 1996 ; Vol. 43, No. 8. pp. 967-969.
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