Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay

Hiroshi Kimura, Makoto Morita, Yumi Yabuta, Kiyotaka Kuzushima, Koji Kato, Seiji Kojima, Takaharu Matsuyama, Tsuneo Morishima

Research output: Contribution to journalArticle

441 Citations (Scopus)

Abstract

To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 107 copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 103.7 copies/μg of DNA in patients with EBV-related lymphoproliferative disorders, 104.1 copies/μg of DNA in patients with chronic active EBV infections, and 102.2 copies/μg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.

Original languageEnglish
Pages (from-to)132-136
Number of pages5
JournalJournal of Clinical Microbiology
Volume37
Issue number1
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Human Herpesvirus 4
Real-Time Polymerase Chain Reaction
Epstein-Barr Virus Infections
DNA
Blood Cells
Viruses
Infectious Mononucleosis
Lymphoproliferative Disorders
Virus Diseases
In Situ Hybridization
Cell Count

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Kimura, H., Morita, M., Yabuta, Y., Kuzushima, K., Kato, K., Kojima, S., ... Morishima, T. (1999). Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. Journal of Clinical Microbiology, 37(1), 132-136.

Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. / Kimura, Hiroshi; Morita, Makoto; Yabuta, Yumi; Kuzushima, Kiyotaka; Kato, Koji; Kojima, Seiji; Matsuyama, Takaharu; Morishima, Tsuneo.

In: Journal of Clinical Microbiology, Vol. 37, No. 1, 01.01.1999, p. 132-136.

Research output: Contribution to journalArticle

Kimura, H, Morita, M, Yabuta, Y, Kuzushima, K, Kato, K, Kojima, S, Matsuyama, T & Morishima, T 1999, 'Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay', Journal of Clinical Microbiology, vol. 37, no. 1, pp. 132-136.
Kimura H, Morita M, Yabuta Y, Kuzushima K, Kato K, Kojima S et al. Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. Journal of Clinical Microbiology. 1999 Jan 1;37(1):132-136.
Kimura, Hiroshi ; Morita, Makoto ; Yabuta, Yumi ; Kuzushima, Kiyotaka ; Kato, Koji ; Kojima, Seiji ; Matsuyama, Takaharu ; Morishima, Tsuneo. / Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. In: Journal of Clinical Microbiology. 1999 ; Vol. 37, No. 1. pp. 132-136.
@article{17e68c2f4ebb45b2a291f217b42c8b33,
title = "Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay",
abstract = "To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 107 copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 103.7 copies/μg of DNA in patients with EBV-related lymphoproliferative disorders, 104.1 copies/μg of DNA in patients with chronic active EBV infections, and 102.2 copies/μg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.",
author = "Hiroshi Kimura and Makoto Morita and Yumi Yabuta and Kiyotaka Kuzushima and Koji Kato and Seiji Kojima and Takaharu Matsuyama and Tsuneo Morishima",
year = "1999",
month = "1",
day = "1",
language = "English",
volume = "37",
pages = "132--136",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay

AU - Kimura, Hiroshi

AU - Morita, Makoto

AU - Yabuta, Yumi

AU - Kuzushima, Kiyotaka

AU - Kato, Koji

AU - Kojima, Seiji

AU - Matsuyama, Takaharu

AU - Morishima, Tsuneo

PY - 1999/1/1

Y1 - 1999/1/1

N2 - To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 107 copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 103.7 copies/μg of DNA in patients with EBV-related lymphoproliferative disorders, 104.1 copies/μg of DNA in patients with chronic active EBV infections, and 102.2 copies/μg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.

AB - To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 107 copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 103.7 copies/μg of DNA in patients with EBV-related lymphoproliferative disorders, 104.1 copies/μg of DNA in patients with chronic active EBV infections, and 102.2 copies/μg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.

UR - http://www.scopus.com/inward/record.url?scp=0032930344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032930344&partnerID=8YFLogxK

M3 - Article

C2 - 9854077

AN - SCOPUS:0032930344

VL - 37

SP - 132

EP - 136

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -