Quantitative analysis of methylglyoxal, glyoxal and free advanced glycation end-products in the plasma of wistar rats during the oral glucose tolerance test

Si Jing Chen, Chiwa Aikawa, Toshiro Matsui

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The purpose of this study was to gain insight into the production behavior of free adducts of advanced glycation end-products (AGEs) in Wistar rats under acute hyperglycemic conditions. Five AGE-free adducts as well as their precursors (i.e., highly reactive carbonyl intermediates of methylglyoxal and glyoxal) in rat plasma were quantitatively determined at greater than nanomolar levels using the liquid chromatography/ tandem mass spectrometry method coupled with 2,4,6-trinitrobenzene sulfonate and 2,3-diaminonaphthalene derivatization techniques. An oral glucose (2 g/kg dose) tolerance test to 10-week-old Wistar rats provided evidence that the plasma levels of diabetes-related metabolites did not change acutely within 120 min, irrespective of increasing blood glucose levels.

Original languageEnglish
Pages (from-to)336-339
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume38
Issue number2
DOIs
Publication statusPublished - Feb 1 2015

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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